采用饲喂法和微注射法评价Cry2Aa蛋白对二化螟盘绒茧蜂的安全性

为评价转Cry2Aa基因水稻T2A-1对二化螟盘绒茧蜂Cotesia chilonis的安全性，采用饲喂法和微注射法综合评价转Cry2Aa基因水稻T2A-1对二化螟盘绒茧蜂的影响。结果表明，寄生饲喂转Cry2Aa基因水稻T2A-1的二化螟Chilo suppressalis后，二化螟盘绒茧蜂体内未检出Cry2Aa蛋白，其每茧块茧数、茧长、雄成虫寿命和雌雄比分别为20.00个、2.58 mm、2.34 d和3.10，均显著小于对照，而卵及幼虫的总历期、蛹期、雌成虫寿命、茧质量和成虫质量均与对照差异不显著；寄生微注射Cry2Aa蛋白的二化螟后，除卵及幼虫的总历期和蛹期外，二化螟盘绒茧蜂其他生命表参数和体内过氧化物酶（peroxidase，POD）、超氧化物歧化酶（superoxide dismutase，SOD）和谷胱甘肽还原酶（glutathione reductase，GR）活力均与阳性对照之间差异显著；寄生微注射Cry2Aa蛋白的二化螟后，二化螟盘绒茧蜂所有生命表参数及体内POD、SOD和GR活力均与阴性对照之间无显著差异。表明饲喂法中转Cry2Aa基因水稻T2A-1对二化螟盘绒茧蜂产生的影响是由寄主介导效应引起的，而非Cry2Aa蛋白本身，因此转Cry2Aa基因水稻T2A-1对二化螟盘绒茧蜂安全。

Safety evaluation of Cry2Aa protein on Cotesia chilonis using feeding and micro-injection methods

To evaluate the safety of transgenic Cry2Aa rice T2A-1 on the parasitoid wasp Cotesia chilonis, a comprehensive assessment was conducted using feeding and microinjection methods. The results revealed that no detectable Cry2Aa protein was found in parasitoid wasps in the rice stem borers Chilo suppressalis fed on transgenic Cry2Aa rice T2A-1. The number of cocoons, cocoon length, male adult lifespan, and sex ratio of C. chilonis fed on T2A-1 rice were significantly lower than those of the control group,with values of 20.00, 2.58 mm, 2.34 d, and 3.10, respectively. However, the total developmental duration for eggs and larvae, the pupal period, female adult lifespan, cocoon weight, and adult weight showed no significant differences from control group. In the case of microinjection of Cry2Aa protein into the rice stem borers, there were significant differences in most life table parameters (except the total developmental duration of egg and larva, and the pupal period), as well as the activities of peroxidase, superoxide dismutase, and glutathione reductase enzymes compared to the positive control. However, there were no significant differences in all life table parameters and enzyme activities when compared to the negative control. These results indicated that the observed effects in the feeding method were mediated by the host-induced effects rather than the Cry2Aa protein itself, suggesting the safety of transgenic Cry2Aa rice T2A-1 on the parasitoid wasp C. chilonis.