EGF·Insulin和IGF-I对食蟹猴耳部成纤维细胞体外增殖的影响

探讨了培养基中不同浓度血清情况下，分别添加不同浓度的表皮生长因子（EGF）、胰岛素（Insulin）和胰岛素样生长因子-I（IGF-I）对食蟹猴耳部成纤维细胞体外增殖的影响，目的是寻找这3种物质促进体外培养成纤维细胞的最佳浓度，改善细胞体外培养系统，为以后体细胞及干细胞培养提供参考。将处于对数生长期的10～15代细胞进行常规消化混匀，计数，接种，培养24h后分别向孔中加入含5％和10％的两种血清浓度、添加不同浓度的EGF、Insulin和IGF-I的培养基，6d后分别向孔中加入MTT，然后用酶联免疫检测仪测定各孔在492nnl处的吸光度值（0D）。血清浓度为10％情况下，培养基中添加10ng／ml EGF、10μg／ml Isulin、100ng／ml IGF-I时，食蟹猴耳部成纤维细胞增殖能力最好。

Effects of EGF, Insulin and IGF-I on the Proliferation of Fibroblast Cells in the Ear of Macaca fascicularis in vitro

The objective of this study was to discuss the effects of supplementation of different concentration of EGF,insulin and IGF-I for cell proliferation of Macaca fascicularis ear fibroblast cells under 5% and 10% FBS concentrations of serum by using colorimetric MTT assay.The results showed that: when the serum concentration was 5% in DMEM,the addition of EGF(20-80 ng/ml),insulin(15 μg/ml) and IGF-I(50-100 ng/ml) had significant promoting effects on the growth of the cells respectively(P<0.05);when the serum concentration was 10% in DMEM,the addition of EGF(10-40 ng/ml),insulin(10-15 μg/ml) and IGF-I(25-100 ng/ml)had significant promotion effects on the growth of the cells respectively(gPg<0.05).