自然侵染菜豆的花生矮化病毒研究

 对从北京郊区菜豆皱缩花叶病株上获得的一个病毒分离物进行了系统研究。根据该分离物的寄主范围、传播特性、病毒颗粒形态、血清学性质和理化特性等,确定为花生矮化病毒(Peanut Stunt Virus,PSV),并称为PSV菜豆分离物,即PSV Bean Isolate,简称PSV-B。这是国内从菜豆上分离PSV的首次报道。在测定的5科28种植物中,PSV-B侵染5科21种。PSV-B致死温度60~65℃,稀释限点10^-4~10^-5,存活期限6 d以上。PSV-B能被豆蚜(Aphis craccivora Koch)以非持久性方式传播。提纯病毒颗粒球形,直径30 nm。制备的PSV-B抗血清,采用琼脂双扩散法测定效价为1 128。在血清学关系上,P SV-B与已报道的中国PSV-Mi株系相近,与美国PSV-E株系差异明显。PSV-B外壳蛋白亚基分子量为25 000道尔顿;含有4个RNA片段。

IDENTIFICATION AND CHARACTERIZATION OF AN ISOLATE OF PEANUT STUNT VIRUS FROM NATURALLY INFECTED BEANS IN CHINA

Abstract A virus isolated from a diseased bean plant in the Beijing suburb was identified as peanut stunt virus, designated PSV Bean isolate (PSV-B) on the basis of host range, transmission characteristics, in vitro properties, virus particle morphology, serology and physical and chemical properties, which was first reported in China. PSV-B infected 21 of 28 species tested in five families. The thermal inactivation point was 60-65. The dilution end point was 10^-4-10^-5 and the longevity in vitro was 6 days. ^{Aphis craccivora} Koch transmitted the virus in a nonpersistent manner. The virus was successfully purified by modified Franck's method for purification of CMV, virus yield was 19.6 mg/100 g infected tissue. Virus particles were spherical with 30 nm in diameter. An antiserum against PSV-B was produced with titers of 1128 in gel diffusion tests. Serologically PSV-B was closely related to PSV-Mi, distantly related to PSV-E. The molecular weight of PSV-B coat protein is about 25 000 D.PSV-B has four kinds of strand RNA.