| 大丽轮枝菌核糖体基因ITS区段的特异扩增 |
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| 引用本文： | 朱有勇,王云月,Bruce R.Lyon.大丽轮枝菌核糖体基因ITS区段的特异扩增[J].植物病理学报,1999,29(3):250-255. |
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| 作者姓名： | 朱有勇 王云月 Bruce R.Lyon |
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| 作者单位： | 1 云南农业大学云南省植物病理重点实验室 昆明 650201;2 澳大利亚悉尼大学生命科学学院分子遗传学实验室 悉尼 2006 |
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| 摘 要： | 根据棉花黄萎病菌(Verticillium dahliae)核糖体基因ITS区段的碱基编码序列,设计合成了1对为26 bp的PCR特异扩增引物(引物1:5'CATCAGTCTCTCTGTTTATACCAACG,和引物2:3'CGATGCGAGCTGTAACTACTACGCAA),进行了大丽轮枝病菌PCR特异扩增试验。试验结果表明:本试验设计合成的这对引物,能对大丽轮枝菌全基因组DNA和人工接种棉花黄萎病病株组织特异地扩增到大丽轮枝菌核糖体基因ITS区段的324 bp分子片段,该对引物可用于棉花黄萎病的分子鉴定和分子监测。本试验结果对棉花黄萎病的早期诊断和病原菌的检测和监测有潜在的应用价值。
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| 关 键 词： | 大丽轮枝菌 特异扩增 分子片段 |
PCR DETECTION OF Verticillium dahliae FROM DISEASED PLANTS |
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| Bruce R.Lyon.PCR DETECTION OF Verticillium dahliae FROM DISEASED PLANTS[J].Acta Phytopathologica Sinica,1999,29(3):250-255. |
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| Authors: | Bruce RLyon |
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| Institution: | 1 Phytopathology Lab. of Yunnan, Kunming 650201;2 School of Biological Sciences. University of Sydney Australia. Sydney 2006 |
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| Abstract: | A pair of PCR primers (26 bp) were designed according to the ITS sequence of rRNA gene of Verticillium dahliae. (P1:5'CATCAGTCTCTCTGTTTATACCAACG, P2:3'CGATGCGAGCTGTAACTACTACGCAA). Results from assays done with these two primers showed that a 324 bp fragment of rRNA gene was amplified from genomic DNA of V. dahliae and diseased plant tissue inoculated with V. dahliae. These two primers will be useful for molecular identification and detection of cotton wilt. Results from this study are very important to the diagnose of cotton wilt by V. dahliae in the early stage,and study of pathogenicity and disease forecast. |
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| Keywords: | Verticillium dahliae PCR primer fragment |
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