柑橘皮中抗过敏活性成分的提取及其抗过敏活性的研究(英文)

目的]该研究旨在确定柑橘皮中抗过敏活性成分提取的最佳工艺条件。方法]本试验对柑橘皮中抗过敏活性成分进行提取,通过单因素试验研究乙醇浓度、提取时间、提取温度、料液比和提取次数等提取条件对提取效果的影响,并通过正交试验优化其提取工艺。结果]通过单因素试验、正交试验,确定了柑橘皮中抗过敏粗提物的最佳提取条件为:提取温度为60℃,乙醇浓度为70%,提取时间为60min,料液比1:25,提取2次。结论]该工艺操作简单、提取效率高、对环境友好,适合柑橘皮中抗过敏活性粗提物的提取。该研究为有效、快速提取柑橘皮中抗过敏成分提供了一条新的方法和研究思路。

Research on Extraction of Anti-allergic Active Ingredient from Citrus Peels and Its Anti-allergic Activity

Objective] This study aimed to determine the optimal process conditions for extraction of anti-allergic active ingredient from citrus peels.Method] Crude anti-allergic active extracts of citrus peels were extracted.Effects of extraction conditions including extraction temperature,ethanol concentration,extraction time and liquid-to-solid ratio on extraction were explored with single-factor experiment,and the extraction process was optimized with orthogonal experiment.Result] Based on the single-factor experiment and orthogonal experiment,the optimal extraction condition for crude anti-allergic active extracts of citrus peels were determined to be under extraction temperature of 60 ℃,ethanol concentration of 70%,extraction time of 60 min,liquid-to-solid ratio of 1:25 and twice extraction.Conclusion] This process condition is of simple operation and high extraction efficiency,which is environment-friendly and suitable for the extraction of crude anti-allergic active extracts from citrus peels.This study provided a new approach and research ideas for the effective and rapid extraction of anti-allergic active ingredient in citrus peels.