同工酶电泳在君子兰分类和育种中的应用

以君子兰不同器官为试材，采用聚丙烯酰胺不连续系统垂直板电泳方法，探讨了酯酶同工酶、多酚氧化酶同工酶和过氧化物酶同工酶在君子兰分类和育种中应用的可行性。结果表明：君子兰植株成熟叶片的过氧化物酶同工酶酶谱清晰稳定，较适宜应用于君子兰。对君子兰不同杂交组合F1过氧化物酶同工酶进行的系统聚类分析显示：油匠×圆头组合F1中，70%倾向于父本，30%发生变异；圆头×油匠组合F1中，有50%倾向于母本，10%倾向于父本；花脸×圆头组合F1中，有70%倾向于母本，20%倾向于父本，10%发生变异。据此可为君子兰的分类和育种提供科学依据。

The applied research of isozyme electrophoresis in Clivia miniata classification and breeding

The paper discussed the feasibility of esterase isozyme，polyphenol oxidase isozyme and peroxidase isozyme utilized in Clivia miniata classification and breeding adopting the polyacrylamide discontinuous system vertical slab electrophoresis technique by using different organs of Clivia miniata as the test materials. The results showed that the peroxidase isozyme pattern of mature leaves of Clivia miniata plants was clear and stable and this method was suitable for the application in Clivia miniata. The results of peroxidase isozyme system cluster analysis among different F1 crossbreds of Clivia miniata showed that 70% F1 tend to paternal，and 30% F1 variation occurred in Oil Carpenter×Round combination；50% F1 tend to parent，and 10% F1 tend to paternal in Round×Oil Carpenter combination；70% F1 tend to parent，20% F1 tend to paternal，and 10% F1 variation occurred in Erhualian×Round combination；Accordingly， the scientific basis was provided for the classification and breeding of Clivia miniata.