恩诺沙星对卤虫无节幼体的急性毒性

为明确恩诺沙星对卤虫无节幼体的急性毒性，设置了0，2，4，6，8，10，11，12，14 mg·mL-1 9个恩诺沙星浓度，分析24 h半最大效应浓度(24 h EC50)、24 h半致死浓度(24 h LC50)和48 h半最大效应浓度(48 h EC50)、48 h半致死浓度(48 h LC50)。结果表明，25℃条件下，24和48 h恩诺沙星对卤虫无节幼体的最大不致死浓度分别为04和02 mg·mL-1，使卤虫无节幼体全部死亡的最低浓度分别为 14 和11 mg·mL-1。采用概率单位法计算恩诺沙星对卤虫无节幼体的EC50和LC50。恩诺沙星对卤虫无节幼体24和48 h的EC50分别为(1066 ± 033)和(1054 ± 033) mg·mL-1，LC50分别为 (946 ± 033)和(771 ± 033) mg·mL-1。结果表明，卤虫无节幼体对恩诺沙星的敏感性较低，常规使用范围内，对卤虫无节幼体的存活无明显影响。

Acute toxicity of enrofloxacin on Artemia nauplius

A serial of concentration gradient of enrofloxacin were used to evaluate the effect of acute toxicity of enrofloxacin on Artemia nauplius. Nine different concentrations of enrofloxacin were tested in the study， which were 0， 2， 4， 6， 8， 10， 11， 12 and 14 mg·mL-1. The 24 h EC50 and 48 h EC50 (effective concentration for 50% reduction)， and 24 h LC50 and 48 h LC50 (lethal concentration for 50%) of enrofloxacin were calculated by the method of probability unit. The results showed that， at 24 h and 48 h， the maximum concentrations of enrofloxacin that could not make Artemia nauplius die were 04 mg·mL-1 and 02 mg·mL-1， respectively. The minimum concentrations that made Artemia nauplius to die entirely were 14 mg·mL-1 and 11 mg·mL-1， respectively. The 24 h EC50 and 48 h EC50 of enrofloxacin to Artemia nauplius were (1066 ± 033) mg·mL-1 and (1054±033) mg·mL-1， respectively. The 24 h LC50 and 48 h LC50 of enrofloxacin to Artemia nauplius were (946 ± 033) mg·mL-1 and (771 ± 033) mg·mL-1， respectively. The results suggested that Artemia nauplius was not sensitive to enrofloxacin.