60Coγ射线种子辐射对大花高代组培苗的生长及诱变效应

以0Gy、10Gy、50Gy、100Gy、150Gy和200Gy不同剂量的60Coγ射线辐射种子，研究其对大花高代组培苗生长影响的结果表明，在50~200Gy范围内，根和真叶的生长以及植株高度都不同程度地受到了抑制，且抑制程度与辐射剂量呈正相关，而10Gy则能促进根和真叶的生长。随着剂量的增高，死亡率增高，组培苗生长41d时的半致死剂量(LD50)为150Gy。各处理组培苗培养70d后，少量瓶内开花，出现花型、花色和花瓣变异。花型变异主要由对照的正常花形变为蝴蝶形。花色变异主要是颜色变浅，甚至变为白色。花瓣变异可由原来的单瓣变为重瓣花。以上结果表明，60Coγ射线辐射可以提高突变频率且诱变效应明显，有利于在大花高代育种中产生色彩丰富，花型、株型美观的变异类型

Effects of Seed Irradiation with 60CoγRay on the Growth and Mutation of Seedling of Godetia grandiflora Lindl. in vitro

Six doses 0Gy, 10Gy, 50Gy, 100Gy, 150Gy and 200Gy were used to study the effects of seed irradiation with 60Coγray on the growth of seedling of Godetia grandiflora Lindl.in vitro in this experiment. The results showed that the number of roots and leaves, and plant height all decreased with the increase of irradiating doses (50-200Gy). Moreover, the higher dose, the higher death rate, and the Half Lethal Dosage (LD50) for the seedlings of 41 days was 150 Gy. Mutants were found amongst seedlings in all treatments 70 days after culturing, including flower colour, shape and the structure of patals. This indicats that gamma ray irradiation can increase the frequency of mutations and produces special types of flower in Godetia grandiflora breeding.