| 冬枣两个乙烯受体编码基因的克隆及序列分析 |
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| 引用本文： | 魏绍冲,彭福田,束怀瑞,孙旭东.冬枣两个乙烯受体编码基因的克隆及序列分析[J].园艺学报,2007,34(2):333-338. |
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| 作者姓名： | 魏绍冲 彭福田 束怀瑞 孙旭东 |
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| 作者单位： | (山东省作物生物学重点实验室, 山东农业大学园艺科学与工程学院, 山东泰安271018) |
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| 基金项目： | 山东农业大学校科研和教改项目 |
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| 摘 要： | 根据植物乙烯受体ETR1家族氨基酸和核苷酸的保守区序列设计简并引物, 通过RT2PCR从半红期冬枣果实中分离了两个1 058 bp的乙烯受体cDNA片段。在分析已知序列基础上, 分别通过3′RACE及对两个基因上游5′端编码区的扩增, 得到了两个包含完整开放阅读框(ORF) 以及3′端非编码区( 3′UTR) 的乙烯受体编码基因, 即ZjETR1和ZjERS1。它们分别编码738个和632个氨基酸。这两个编码基因的氨基酸与其它植物乙烯受体氨基酸高度同源, 分别属于ETR1亚类和ERS1亚类乙烯受体。
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| 关 键 词： | 枣 乙烯受体 果实成熟 序列分析 基因克隆 |
| 文章编号： | 0513-353X（2007）02-0333-06 |
| 收稿时间： | 2006-06-12 |
| 修稿时间： | 2006-06-122006-12-28 |
Cloning and Sequence Analysis of Two Encoding Ethylene Receptor cDNAs in 'Dongzao' Jujube |
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| WEI Shao-chong,PENG Fu-tian,SHU Huai-rui,SUN Xu-dong.Cloning and Sequence Analysis of Two Encoding Ethylene Receptor cDNAs in ''''Dongzao'''' Jujube[J].Acta Horticulturae Sinica,2007,34(2):333-338. |
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| Authors: | WEI Shao-chong PENG Fu-tian SHU Huai-rui SUN Xu-dong |
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| Institution: | ( Key Laboratory of Crop Biology of Shandong Province, College of Horticulture Science and Engineering, Shandong Agricultural University, Taipan, Shandong 271018, China) |
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| Abstract: | Two cDNA fragments with 1 058 bp were amplified from Chinese jujube(Zizyphus jujuba 'Dongzao')fruit at a half-red mature stage through RT-PCR using degenerated primers,and then both 5'encoding region and 3'-end with a UTR(untranslated region)of these two cDNA fragment sequences were obtained.The obtained ethylene receptor genes with two open reading frames,referred to ZjETR1 and ZjERS1,encoded 738 and 632 amino acids,respectively.Sequence analysis indicated that ZjETR1 and ZjERS1 shared a high similarity with other ethylene receptors at the polypeptide level,and could be divided into the ETR1 and ERS1 sub-groups. |
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| Keywords: | Jujube Ethylene receptor Fruit ripening Sequence analysis Gene cloning |
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