Influence of rice-isolated bacteria on chitinase production by the biocontrol bacterium Serratia marcescens strain B2 and the genetically modified rice epiphytic bacterium |
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Authors: | Nobutaka Someya Shinichi Numata Masami Nakajima Akira Hasebe Katsumi Akutsu |
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Institution: | (1) School of Agriculture, Ibaraki University, Ami-machi, Ibaraki 300-0393, Japan;(2) National Institute for Agro-Environmental Sciences, Tsukuba, Japan;(3) Present address: RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Yokohama, Japan |
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Abstract: | Chitinolytic activity of the biocontrol bacterium Serratia marcescens strain B2 was inhibited by bacteria isolated from rice, even though its growth was not affected. Antifungal activity of the strain against Pyricularia oryzae was also reduced under the influence of these bacteria. In contrast, the rice-epiphytic bacterium Erwinia ananas NR1, transformed with chitinase gene chiA derived from strain B2, had high chitinolytic activity regardless of the presence of the bacteria isolated from rice. Therefore, the introduction of an antagonistic factor gene with a promoter from the recipient into the epiphytic bacteria may prove useful in the development of effective biocontrol agents. |
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Keywords: | Biological control Chitinolytic enzyme Erwinia ananas Genetically modified microorganism Indigenous microorganism Serratia marcescens |
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