| 濒危黑颈长尾雉圈养种群的RAPD遗传多样性分析 |
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| 引用本文： | 庾太林,韩增超,管清新,张良建,刘晓辉. 濒危黑颈长尾雉圈养种群的RAPD遗传多样性分析[J]. 广西农业生物科学, 2012, 0(4): 369-373 |
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| 作者姓名： | 庾太林 韩增超 管清新 张良建 刘晓辉 |
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| 作者单位： | 广西师范大学生命科学学院,桂林541004 |
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| 基金项目： | 本研究由国家自然基金项目（No.31160426）资助 |
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| 摘 要： | 运用RAPD技术对黑颈长尾雉圈养种群的遗传多样性进行了分析。从50条随机引物中筛选出14条引物，对24个个体的基因组DNA进行了PCR扩增，从检测出的119个位点中有98个多态位点，占总位点的82．35％，标记的分子量大小范围是0．2～3kb。24个个体问的遗传距离幅度0．1597～0．4874，平均是0．2810；用软件NTsys2．10e构建了24个个体相互关系的分支图，24个个体可分为3个类群。实验表明：黑颈长尾雉圈养种群的遗传多样性水平较高，圈养种群内遗传差异性较大。
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| 关 键 词： | 黑颈长尾雉 圈养种群 RAPD 遗传多样性 |
Genetic Diversity of the Domesticated Population of Endangered Syrmaticus humiae Revealed by RAPD Amplification |
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| Yu Tailin Han Zengchao Guan Qingxin Zhang Liangjian Liu Xiaohui. Genetic Diversity of the Domesticated Population of Endangered Syrmaticus humiae Revealed by RAPD Amplification[J]. Journal of Guangxi Agricultural and Biological Science, 2012, 0(4): 369-373 |
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| Authors: | Yu Tailin Han Zengchao Guan Qingxin Zhang Liangjian Liu Xiaohui |
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| Affiliation: | Yu Tailin Han Zengchao Guan Qingxin Zhang Liangjian Liu Xiaohui College of Life Science, Guangxi Normal University, Guilin, 541004 |
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| Abstract: | The genetic diversity of cultured stocks of Syrmatic us humiae has been studied by RAPD technique. 24 individuals DNA were used for genetics analysis and 14 of 50 random primers have been selected for PCR amplification. Among the amplified 119 loci, 98 loci were polymorphic which occupied 82.35% in total. The molecular weights were from 0.2 kb to 3 kb. The genetic distance among 24 individuals ranged from 0.159 7 to 0.487 4 and the average genetic distance was 0.281 0. After the dendrogram was constructed by NTsys 2.10e software, the 24 individuals ofSyrmaticus humiae could be separated into 3 major clusters. In conclusion, there are higher genetic diversity and differentiation ofSyrmaticus humiae in captivity population. |
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| Keywords: | Syrmaticus humiae Domesticated population RAPD Genetic diversity |
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