| 玉米抗矮花叶病毒B株系的QTL定位 |
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| 引用本文： | 刘小红,何道文,张红伟,张红梅,谭振波,荣廷昭.玉米抗矮花叶病毒B株系的QTL定位[J].河北农业大学学报,2006,29(6):56-59. |
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| 作者姓名： | 刘小红 何道文 张红伟 张红梅 谭振波 荣廷昭 |
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| 作者单位： | 西华师范大学,生命科学学院,四川,南充,637002;北京大北农,农业科技研究院,北京,100085;四川农业大学,玉米研究所,四川,雅安,625014;西华师范大学,生命科学学院,四川,南充,637002;北京大北农,农业科技研究院,北京,100085;四川农业大学,玉米研究所,四川,雅安,625014 |
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| 基金项目： | 北京市海淀科技发展计划项目
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西华师范大学校科研和教改项目 |
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| 摘 要： | 为明确玉米对矮花叶病的抗病机制,以代表国内外两大玉米杂种优势类群的优良自交系黄早4和Mo 17为亲本,构建了含239个重组自交系的F9代分离群体,并利用该群体构建了包含101个SSR标记的遗传连锁图谱,图谱全长1422.7 cM,标记间的平均图距为15.6 cM。通过人工接种病毒鉴定,评价了亲本及群体对玉米矮花叶病毒B株系的抗性反应。采用复合区间作图法对玉米矮花叶病抗性QTL进行了定位及其遗传效应分析,在第5、6染色体上,各定位了控制发病率的1个微效QTL和1个主效QTL,分别与标记Bnlg602和Bnlg161连锁,其遗传效应能分别解释表型方差的2.3%和33.8%。
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| 关 键 词： | 玉米 重组自交系 数量性状位点 玉米矮花叶病毒B株系 |
| 文章编号： | 1000-1573（2006）06-0056-04 |
| 收稿时间： | 2006-04-16 |
| 修稿时间： | 2006-04-16 |
QTL mapping for resistance to MDMV-B in maize |
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| LIU Xiao-hong,HE Dao-wen,ZHANG Hong-wei,ZHANG Hong-mei,TAN Zhen-bo,RONG Ting-zhao.QTL mapping for resistance to MDMV-B in maize[J].Journal of Agricultural University of Hebei,2006,29(6):56-59. |
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| Authors: | LIU Xiao-hong HE Dao-wen ZHANG Hong-wei ZHANG Hong-mei TAN Zhen-bo RONG Ting-zhao |
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| Abstract: | In order to realize the mechanism of resistance for maize to MDMV-B,quantitative trait loci(QTLs) for resistance to MDMV-B was mapped and analyzed,based on a maize F_9 population consisting of 239 recombinant inbred lines(RILs) derived from the cross of Huangzao 4(resistant) and Mo 17(susceptible).The genetic linkage map was constructed including 101 SSR markers,covering 1422.7 cM in maize genome with an interval length of 15.6 cM.The parents and their RILs were evaluated on resistance to MDMV-B in field trials via artificial inoculation.The method of composite interval mapping was employed for QTL detection,two QTLs for percentage of disease symptom plants,linked with markers bnlg602 and bnlg161,were identified on chromosome 5 and 6,which can explain 2.3% and 33.8% of the phenotypic variance,respectively. |
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| Keywords: | maize(Zea mays L ) recombinant inbred line(RIL) quantitative trait locus(QTL) maize dwarf mosaic virus strain B(MDMV-B) |
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