| 红千层愈伤组织诱导及植株再生 |
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| 引用本文： | 伍成厚,冯毅敏,叶振华,朱纯,龙丽萍.红千层愈伤组织诱导及植株再生[J].经济林研究,2007,25(3):11-14. |
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| 作者姓名： | 伍成厚 冯毅敏 叶振华 朱纯 龙丽萍 |
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| 作者单位： | 1. 广州市园林科学研究所,广东,广州,510405;厦门大学,生命科学学院,福建,厦门,361005
2. 广州市园林科学研究所,广东,广州,510405 |
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| 基金项目： | 广东省科技厅科技计划
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广东省广州市科技计划 |
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| 摘 要： | 以茎段、芽和叶片为材料,研究了红千层愈伤组织诱导及植株再生的方法。结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养愈伤组织可发育成绿色和粉红色2种类型,其中绿色、致密的愈伤组织可以分化出不定芽。培养基1/2M S 6-BA 1.0 m g/L NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S IBA 0.25 m g/L上试管苗的生根率可达95%。
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| 关 键 词： | 红千层 愈伤组织诱导 植株再生 组织培养 |
| 文章编号： | 1003-8981（2007）03-0011-04 |
| 收稿时间： | 2007-03-07 |
| 修稿时间： | 2007年3月7日 |
Callus Induction and Plant Regeneration in Callistemon rigidus |
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| WU Cheng-hou,FENG Yi-min,YE Zhen-hua,ZHU Chun,LONG Li-ping.Callus Induction and Plant Regeneration in Callistemon rigidus[J].Economic Forest Researches,2007,25(3):11-14. |
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| Authors: | WU Cheng-hou FENG Yi-min YE Zhen-hua ZHU Chun LONG Li-ping |
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| Institution: | 1. Guangzhou Institute of Landscape and Garden, Guangzhou 510405, Guangdong, China; 2. School of Life Science, Xiamen University, Xiamen 361005, Fujian, China |
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| Abstract: | Taken stem segment,bud and leaf as materials,the means of callus induction and plant regeneration in Callistemon rigidus R.Br.were studied.The results showed that callus could be induced from all types of the materials,pink and green callus could be gain through subculture,and adventitious buds could be induced from the compacted greens.The optimal medium for inducing adventitious bud was 1/2MS supplemented with 6-BA1.0 mg/L and NAA0.1 mg/L,and the optimal one for test-tube plantlets rooting was 1/2MS supplemented with IBA0.25 mg/L,in which the rooting rate could be up to 95%. |
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| Keywords: | Callistemon rigidus R Br callus induction plant regeneration tissue culture |
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