| 应用实时荧光PCR检测苹果果实球壳孢腐烂病菌 |
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| 引用本文： | 邓丛良,边勇,梁新苗,吕玉峰.应用实时荧光PCR检测苹果果实球壳孢腐烂病菌[J].植物检疫,2012(1):29-31. |
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| 作者姓名： | 邓丛良 边勇 梁新苗 吕玉峰 |
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| 作者单位： | 北京出入境检验检疫局检验检疫技术中心 |
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| 基金项目： | 国家公益性课题(20111035);国家973基金资助(2011CB932803);国家质检总局课题(2011IK157) |
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| 摘 要： | 根据苹果果实球壳孢腐烂病菌(Sphaeropsis pyriputrescens Xiao&J.D.Rogers)ITS区序列设计特异性引物和TaqMan荧光探针,建立了苹果果实球壳孢腐烂病菌的实时荧光PCR检测方法。该方法能够特异性检测苹果果实球壳孢腐烂病菌,供试的目标菌株检测结果为阳性,而苹果轮纹病菌、苹果炭疽病菌、苹果干腐病菌和苹果褐腐病菌、苹果腐烂病菌等5种对照菌株检测结果为阴性。该方法具有快速、简便、准确的优点,适合于该病害的快速诊断和口岸检验检疫运用。
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| 关 键 词： | 苹果果实球壳孢腐烂病菌 实时荧光PCR 检测 |
Application of real-time PCR for detecting Sphaeropsis pyriputrescens |
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| Deng Congliang,Bian Yong,Liang Xinmiao,Lv Yufeng.Application of real-time PCR for detecting Sphaeropsis pyriputrescens[J].Plant Quarantine,2012(1):29-31. |
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| Authors: | Deng Congliang Bian Yong Liang Xinmiao Lv Yufeng |
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| Institution: | (Plant Laboratory of Beijing Inspection and Quarantine Testing Center,Beijing 101312,China) |
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| Abstract: | A pair of primers and a TaqMan probe basing on the conserved nucleotide sequence of ITS gene of Sphaeropsis pyriputrescens were designed and synthesized,a novel real-time fluorescent PCR was established to detect Sphaeropsis pyriputrescens.This method was capable of detecting Sphaeropsis pyriputrescens specificly.The detection result of mycelium of Sphaeropsis pyriputrescens by real-time fluorescent PCR showed positive,while that of Botryosphaeria berengeriana f.sp.piricola,Colletotrichum gloeosporioides,Botryosphaeria berengeriana,Sclerotinia fructigena and Nectria galligena showed negative.The reliable,quick and easy-handling detection method is suitable for disease diagnosis and quarantine detection. |
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| Keywords: | Sphaeropsis pyriputrescens realtime PCR detection |
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