巴西橡胶树伤害诱导蛋白基因的克隆及表达分析

为了研究橡胶树在逆境胁迫下的分子机制，根据巴西橡胶树抑制消减(SSH)文库和NCBI数据库中的伤害诱导蛋白基因拼接序列设计嵌套引物，通过RACE技术获得基因的完整开放阅读框，命名为HbWUN1。序列分析表明HbWUN1长872 bp，含有390 bp的开放阅读框，编码129个氨基酸。生物信息学分析结果表明HbWUN1不含信号肽和跨膜结构，为亲水性蛋白。进化分析结果显示，橡胶树HbWUN1与拟南芥NP_974279.1聚成一类。RT-PCR分析结果表明HbWUN1表达无组织特异性，在胶乳中表达量最高。在叶片不同发育时期HbWUN1表达存在变化，在衰老期最高。此外，低温、伤害、高盐、乙烯和茉莉酸处理均调控HbWUN1表达，推测其可能在橡胶树逆境反应中发挥作用。

Cloning and expression analysis of a wound-induced protein gene in Hevea brasiliensis

The aim was to study the molecular mechanism of rubber tree in stress response. The first wound-induced protein gene in Hevea brasiliensis designated as HbWUN1, was isolated according to assembled sequences of two ESTs （GenBank: GO349116 and EC607122）. The complete cDNA of HbWUN1 was 872 bp in size with a 390 bp ORF encoding 129 amino acid. As a predicted hydrophilic protein, no signal peptides and transmembrane structures were predicted within HbWUN1. The phylogenetic analysis indicated that HbWUN1 was clustered together with Arabidopsis thaliana （NP₉74279.1）. The expression pattern of HbWUN1 was no tissue-specific with high expression in latex. In different developing stages of rubber tree leaves, HbWUN1 showed a variation expression profile and reached the highest level at senescence stage. In addition, low temperature, wounding, high salt, ET and JA treatments regulated the expression of HbWUN1, suggesting that HbWUN1 might play vital roles in stress response of rubber tree.