利用SSR分子标记鉴定杂交水稻真伪与纯度

目的]寻找快速有效鉴定杂交水稻种子真伪与纯度的方法，以提高鉴定的效率。方法]利用SSR分子标记鉴定杂交水稻种子中优88、协优88和全丰优88的真伪和纯度，并将鉴定结果与田间鉴定结果进行比较。结果]杂交种的DNA带谱中均含有两个亲本的互补带，说明杂交种为真种子。中优88的SSR分子标记和田间鉴定的纯度分别为97．43％和97．60％；协优88的SSR分子标记和田间鉴定的纯度分别为98．00％和98．10％，全丰优88的SSR分子标记和田间鉴定的纯度分别为96．29％和96．60％，3个杂交种的SSR分子标记鉴定和田间鉴定的结果基本上一致，没有明显的差异，说明用SSR检测杂交水稻种子纯度是完全可行的。结论]SSR分子标记在杂交水稻种子真伪与纯度鉴定上具有较好的应用价值和前景。

Identification of True or False and Purity of Hybrid Rice with SSR Molecular Marker

Objective] The aim was to search for the quick and effective method for the identification of true or false and purity of hybrid rice seeds so as to improve the identification efficiency.Method] The true or false and purities of seeds of the hybrid rice Zhongyou 88,Xieyou 88 and Quanfengyou 88 were identified with SSR molecular marker and the identification result was compared with the field identification result.Result] Two complementary bands of the parents appeared in the DNA band spectra of the hybri...