| 利用启动子捕获技术构建橡胶炭疽病菌突变体库 |
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| 引用本文： | 魏小慧,林春花,郑肖兰,张新春,贺春萍,郑服丛.利用启动子捕获技术构建橡胶炭疽病菌突变体库[J].热带作物学报,2009,30(6):804-810. |
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| 作者姓名： | 魏小慧 林春花 郑肖兰 张新春 贺春萍 郑服丛 |
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| 作者单位： | 1. 海南大学环境与植物保护学院,海南儋州,571737;中国热带农业科学院环境与植物保护研究所,海南儋州,571737
2. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737 |
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| 基金项目： | 国家科技支撑计划课题，热作病虫害疫情监测与防治项目，中央公益性科研院所基本科研业务费专项 |
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| 摘 要： |
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| 关 键 词： | 橡胶炭疽病菌 启动子捕获 农杆菌 遗传转化 |
Mutant Library Constructed by Using Promoter trapping in Colletotrichum gloeosporioides |
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| Wei Xiaohui,Lin Chunhu,Zheng Xiaolan,Zhang Xinchun,He Chunping and Zheng Fucong.Mutant Library Constructed by Using Promoter trapping in Colletotrichum gloeosporioides[J].Chinese Journal of Tropical Crops,2009,30(6):804-810. |
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| Authors: | Wei Xiaohui Lin Chunhu Zheng Xiaolan Zhang Xinchun He Chunping and Zheng Fucong |
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| Institution: | Wei Xiaohui1,2,Lin Chunhua2,Zheng Xiaolan2,Zhang Xinchun2,He Chunping2,Zheng Fucong1,2 1 College of Environment , Plant Protection,Hainan University,Danzhou,Hainan 571737,2 Environment , Plant Protection Institute,CATAS,Danzhou Hainan,571737,China |
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| Abstract: | Promoter -trapping vectors, designated as pCAHPH, were constructed and transformed into Collectotrichum gloeosporioides via ATMT (Agrobacterium tumefaciens-mediated transformation). A library of 1 105 transformants resistant to hygromycin B was generated. Of all the transformants, 248 transformants were randomly selected for detection, of which 19 transformants were reduced greatly in pathogenicity. PCR amplification of randomly selected 7 transformants showed that they were all resistant to hygromycin B an... |
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| Keywords: | promoter trapping Colletotrichum gloeosporioides Agrobacterium tumefaciens-mediated transformation |
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