| 小麦远缘杂交后代的高分子量谷蛋白亚基变异机制探讨 |
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| 引用本文： | 耿惠敏,李勇慧,刘雪琴,押辉远,李锁平.小麦远缘杂交后代的高分子量谷蛋白亚基变异机制探讨[J].河南农业科学,2010(2). |
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| 作者姓名： | 耿惠敏 李勇慧 刘雪琴 押辉远 李锁平 |
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| 作者单位： | 1. 洛阳师范学院,生命科学系,河南,洛阳471022
2. 河南大学,生命科学学院,河南,开封,475001 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 以普通小麦川农19(N,7+8,2+12)和1BL/1RS易位系小麦川农18(1,7+9,2+12)F1为试材,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulphate-polyacrylamide gel elec-trophoresis,SDS-PAGE)法鉴定分析了杂交后代的高分子量谷蛋白亚基组成,发现其中1粒产生了2个亲本中都没有的高分子量谷蛋白亚基,其高分子量谷蛋白亚基组成为(1,x+7+8+9,2+12)。变异的亚基在SDS-PAGE电泳图谱上位于1 Dx5和1 Bx7亚基之间,迁移率与1 Bx6相似。统计变异株F2代HMW-GS的遗传规律,结果表明,Glu-A1位点上的亚基分离正常;而Glu-B1位点上亚基的分离以及Glu-A1与Glu-B1位点之间的组合都不符合孟德尔遗传规律;变异亚基与1 By8亚基呈现共显性。最后对变异产生的机制进行了探讨。
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| 关 键 词： | 小麦 杂交后代 高分子量谷蛋白亚基 变异 |
Variation Mechanism of HMW-GS in Progenies of Wheat Distant Hybridization |
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| GENG Hui-min,LI Yong-hui,LIU Xue-qin,YA Hui-yuan,LI Suo-ping.Variation Mechanism of HMW-GS in Progenies of Wheat Distant Hybridization[J].Journal of Henan Agricultural Sciences,2010(2). |
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| Authors: | GENG Hui-min LI Yong-hui LIU Xue-qin YA Hui-yuan LI Suo-ping |
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| Abstract: | The compositions of high-molecular-weight glutein subunits(HMW-GS) of F1 grains derived from wheat Chuannong19(N,7+8,2+12)×Chuannong18(1,7+9,2+12) were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE),and a HMW-GS variant was detected in one of the grains tested.The HMW-GS patterns of the variant seed were(1,x+7+8+9,2+12).SDS-PAGE analysis showed that the band of variant subunit was similar to 1Bx6,which located between 1Dx5 and 1Bx7.The HMW-GS subunits encoded by Glu-B1 and the combination of Glu-A1 and Glu-B1 loci deviated the Mendel's segregation ratios in F2 progenies.Furthermore,the variant subunit and the 1By8 were codominant.The forming mechanism of the variant HMW-GS was discussed at last. |
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| Keywords: | Wheat Hybridization progeny HMW-GS Variation |
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