| 正交实验法优化沙棘黄酮的提取工艺 |
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| 引用本文： | 侯霄.正交实验法优化沙棘黄酮的提取工艺[J].国际沙棘研究与开发,2010,8(3):21-24,36. |
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| 作者姓名： | 侯霄 |
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| 作者单位： | 山西省医药与生命科学研究院中药室,山西太原,030006 |
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| 基金项目： | 山西省科技攻关资助项目 |
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| 摘 要： | 本文探讨了沙棘果中沙棘总黄酮的最佳提取工艺条件。采用L16（4^5）正交试验设计，以芦丁的提取含量为评价指标，优化沙棘黄酮的醇提工艺。结果表明，沙棘黄酮的醇提最佳工艺是用50％乙醇回流提取3次，第1次用10倍量50％乙醇提取2h，第2次用10倍量50％乙醇提取1h，第3次用8倍量50％乙醇提取1h。乙醇浓度、提取次数对该提取效果具有显著性影响，本提取工艺合理可行。
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| 关 键 词： | 沙棘 黄酮 提取工艺 正交试验 |
On Optimun Extraction Technics of Total Flavonoids from Hippophae rhamnoides L.Fruits by Orthogonal Design |
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| Authors: | Hou Xiao |
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| Institution: | Hou Xiao (Department of the Traditional Chinese Medicine, Institute of Medicine and Life Science of Shanxi Province, Taiyuan 030006, China) |
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| Abstract: | This article were studied the optimum conditions of extracting total flavonoids from Hippo- phae rharnnoides fruits. On index of rutin , the optimum technics was investigated by the L16 （4^5） orthogonal experiment. Results show, the optimum conditions of alcohol extraction were 50% ethanol for two times, with the solvent 10 folds amount and refluxing for 2, lhour; andthen with the 8 fold ethanol refluxing four 1 hour. The concentration of ethanol and extraction times significantly affected the extracting technology. The optimized extraction conditions are scientific , reasonable and stable. |
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| Keywords: | seabuckthorn fruits total flavonoidsl extraction process orthogonal experiment |
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