Diagnosis and molecular analysis of Potato leafroll virus isolates in Tunisia |
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| Authors: | F. Djilani Khouadja,S. Guyader,F. Gorsane,N. Khamassy,J. Rouzé ,M. Marrakchi, H. Fakhfakh |
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| Affiliation: | Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Facultédes Sciences de Tunis, Campus Universitaire El Manar, 2092 Tunis (Tunisie);e-mail: or; Laboratoire de Zoologie UMR BiO3P, INRA Le Rheu, Domaine de la Motte, BP 35327, 35653 Rennes (France); Institut National de la Recherche Agronomique de Tunisie, Ariana (Tunisie) |
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| Abstract: | Potato leafroll virus (PLRV) is a major constraint to potato production in North Africa. Serological (sandwich and cocktail ELISA) and molecular (RT-PCR) tests were used to detect PLRV in 131 potato samples collected in different areas of Tunisia. RT-PCR proved to be usable as a routine diagnostic test for epidemiological purposes, being more sensitive and reliable, and less time-consuming, than serological tests. One RT-PCR-amplified portion of ORF3 (336 nt) was cloned and sequenced, and used for molecular characterization of Tunisian PLRV isolates. These showed high sequence identity with PLRV retrieved from GenBank. |
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