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桉树外植体诱导褐变原因初探
引用本文:李海玲,朱艳. 桉树外植体诱导褐变原因初探[J]. 湖北林业科技, 2007, 0(5): 20-21,67
作者姓名:李海玲  朱艳
作者单位:广西生态工程职业技术学院,柳州,545004
摘    要:桉树外植体诱导产生褐变的因子很多,在此只讨论不同无性系、不同木质化程度的茎段、不同的消毒处理、不同的光照对外植体褐变的影响。从试验结果得出:不同的无性系外植体产生褐变程度不同,采集半木质化或刚木质化的茎段,用0.1%HgCl2分两步消毒可以减轻褐变,在培养过程中采用全暗光的方法有效控制褐变,萌芽率也高。

关 键 词:桉树  组织培养  外植体  褐变
收稿时间:2007-05-19
修稿时间:2007-05-19

Initial Discussion on Reason of Induction Brown - turning outside Eucalyptus Plant Body
Li Hailing,Zhu Yan. Initial Discussion on Reason of Induction Brown - turning outside Eucalyptus Plant Body[J]. Hubei Forestry Science and Technology, 2007, 0(5): 20-21,67
Authors:Li Hailing  Zhu Yan
Affiliation:Guangxi Ecological Engineering Professional Technology Institute Liuzhou 545004
Abstract:There are many factors causing brown-turning on induction outside Eucalyptus plant body, here, only discusses different clone, different stem section of lignifications degree, different disinfection processing and influence of different illumination to brown-turning outside plant body. The result is obtained from the test that different clone outside plant body to cause different brown-turning degree, gather stem section of half or just lignifications to reduce brown-turning using 0.1%HgCl2 to disinfect by two steps, adopts method of entire obscure ray to control effectively and germinating rate is also high during breeding process.
Keywords:Eucalyptus  tissue culture  outside plant body  brown-turning  
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