| 大蒜组织培养快繁技术的研究 |
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| 引用本文： | 胡小京,耿广东,王建龙. 大蒜组织培养快繁技术的研究[J]. 长江蔬菜, 2011, 0(20): 13-15. DOI: 10.3865/j.issn.1001-3547.2011.20.003 |
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| 作者姓名： | 胡小京 耿广东 王建龙 |
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| 作者单位： | 贵州大学农学院,贵阳,550025 |
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| 基金项目： | 科技部、财政部“富民强县”专项行动计划（205）; 贵州农业科技攻关计划项目（黔科合NY字[2009]3020） |
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| 摘 要： | 以贵州务川紫皮大蒜茎尖为试验材料,将其接种在附加不同NAA和6-BA的MS分化培养基中,比较组织诱导、分化和生根效果。试验结果表明,以MS＋6-BA 3.0 mg/L＋NAA 0.5 mg/L培养基诱导愈伤组织效果最好;以MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L培养基诱导不定芽增殖的效果较好,增殖倍数达5倍;以MS＋NAA 0.4 mg/L培养基的生根效果最佳。
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| 关 键 词： | 大蒜 茎尖 组织培养 快繁 |
Research on Tissue Culture and Rapid Propagation Technology of Garlic |
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| HU Xiaojing,GENG Guangdong,WANG Jianlong. Research on Tissue Culture and Rapid Propagation Technology of Garlic[J]. Journal of Changjiang Vegetables, 2011, 0(20): 13-15. DOI: 10.3865/j.issn.1001-3547.2011.20.003 |
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| Authors: | HU Xiaojing GENG Guangdong WANG Jianlong |
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| Affiliation: | (Agricultural College,Guizhou University,Guiyang 550025) |
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| Abstract: | The shoot tip culture of purple garlic from Wuchuan county in Guizhou province was performed on MS media supplemented with different concentration of NAA and 6-BA,to compare their effects on tissue induce,differentiation,rooting.The results showed that the most effective medium to induce callus was MS＋BA 3.0 mg/L＋NAA 0.5 mg/L.The most effective medium to induce adventitious buds was MS＋BA 2.0 mg/L＋NAA 0.1 mg/L,with the proliferation multiple of 5.The medium of MS＋NAA 0.4 mg/L was the best for the root induction. |
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| Keywords: | Garlic Shoot tip Tissue culture Rapid propagation |
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