Relationship between resistance to pine wilt disease and the migration or proliferation of pine wood nematodes |
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Authors: | Yasuhiro Mori Fumihiko Miyahara Yuji Tsutsumi Ryuichiro Kondo |
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Institution: | (1) Fukuoka Prefecture Forest Research and Extension Center, 1438-2, Toyoda Yamamoto-machi, Kurume, Fukuoka 839-0827, Japan;(2) Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan |
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Abstract: | To study the relationship between resistance to pine wilt disease and the migration or proliferation of pine wood nematodes
(PWN) (Bursaphelenchus xylophilus), we conducted experiments using clonally-propagated Japanese black pines (Pinus thunbergii) with pre-evaluated individual resistance levels. Bark including the cortical resin canals—one of the main migration pathways
of PWN—was removed by girdling, but neither the migration of PWN nor symptom development of pine wilt disease were inhibited
by this treatment in non-resistant clones. Histological observations showed no significant differences in the lumen area or
the number of cortical- and xylem- axial resin canals between resistant and susceptible clone groups from a half-sib family.
A bioassay using methanol extracts from resistant and susceptible clones showed that extracts from both clones showed similar
attractant effects to PWN, but neither had repellent effects. The resistant clones were multi-inoculated with PWN into three
split points to mimic migration in the stem. The proportion of damaged plants was not significantly different from that in
single-inoculated plants (control). In this experiment, the number of PWN detected from partially-damaged plants was much
higher than that from non-damaged plants. An inoculation test using stem cuttings showed that the population of PWN increased
in susceptible cuttings at 1–20 days after inoculation (dai), while it remained unchanged or gradually decreased in resistant
cuttings. These findings suggested that the factors contributing to resistance were associated with inhibiting the proliferation
of PWN, rather than inhibiting their migration. |
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Keywords: | Bursaphelenchus xylophilus Clonal propagation Pine wilt disease Pinus thunbergii Resistance |
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