大孔树脂纯化金银花总黄酮及其对α-葡萄糖苷酶抑制活性的研究

为进一步开发和利用金银花资源,筛选出降血糖的活性成分,比较了五种大孔树脂对金银花总黄酮的静态吸附-解吸性能,优选出NKA-2大孔树脂,并对其动态的纯化工艺条件进行探讨;采用不同极性有机溶剂对NKA-2大孔树脂纯化后的金银花总黄酮进行萃取,测定了各相萃取物对α-葡萄糖苷酶的抑制活性。结果表明:NKA-2大孔树脂优化的动态工艺参数为上样液质量浓度1.12 mg/m L、上样液体积50 m L、上样流速3 BV/h、洗脱剂乙醇体积分数70%、洗脱液体积160 m L、洗脱流速3 BV/h;在此优化条件下,金银花总黄酮的平均纯度和平均得率分别达到86.3%和5.12%;金银花总黄酮各相萃取物质量浓度与α-葡萄糖苷酶的抑制率具有正相关性,但是在相同质量浓度下,乙酸乙酯相萃取物对α-葡萄糖苷酶具有最强的抑制率。经过NKA-2大孔树脂纯化后的金银花总黄酮,其乙酸乙酯相萃取物具有良好的药用开发价值。

Purification of Total Flavonoids from Flos Lonicerae by the Macroporous Resins and Its Inhibitory Activity on α-glucosidase

In order to fully develop and utilize Flos Lonicerae resource and screen out active ingredients for hypoglycemic treatment, this paper optimized the NKA-2 macroporous resin from 5 species macroporous resin by comparing their static adsorption and desorption performances on total flavonoids of Flos Lonicerae; and disscussed its dynamic purification process conditions. Then, the paper extracted the total flavonoids after purification with NKA-2 macroporous resin by using organic solvents with different polarities, and determined α-glucosidase inhibitory activities of various extractants. The results showed that: The optimal purification conditions of NKA-2 macroporous resin were 1.12 mg/mL mass concentration of sample solution, 50 mL sample volume, 3 BV/h flow rate, 70% eluent ethanol volume fraction, 160 mL effluent volume, 3 BV/h eluent rate. Under these optimized conditons the mean purity and yield of total flavonoids were up to 86.3% and 5.12%, respectively. There was a positive correlation between mass concentrations of solvent extracts and inhibition rate of α-glucosidase. However, ethyl acetate-soluble fraction still had the strongest α-glucosidase inhibitory activity under the same mass concentrations. The ethyl acetate-soluble fraction from total flavonoids purified by NKA-2 macroporous resin showed good medicine values.