不同烤烟品种(系)叶片组织结构、腺毛及分泌物比较分析

为了探究不同烤烟品种(系)叶片组织结构差异、腺毛发育及腺毛分泌物代谢特点,应用扫描电镜、数字显微镜及气相色谱质谱连用技术,对高香气品种(系) 8306、豫烟11号等8种烤烟品种(系)的叶片组织结构、腺毛密度及腺毛分泌物进行比较研究。结果表明:(1)长柄腺毛是优势腺毛。上表皮腺毛普遍多于下表皮腺毛。豫烟11的短柄腺毛密度、8306的长柄腺毛密度、K326的保护毛密度分别较其他烤烟品种高;西柏三烯二醇是腺毛分泌物组分中的主要化学成分,赖百当化合物包括冷杉醇和赖百当二醇,仅在豫烟11、8306、12451中少量存在,在其他烤烟品种(系)中均未发现;(3)栅栏组织/海绵组织、栅栏组织厚/叶厚、腺毛密度及腺毛分泌物之间存在显著正相关性。高香气烤烟品种(系)的叶片组织结构、腺毛密度、腺毛分泌物总量均显著优于其他品种。

Comparison Analysis of Leaf Tissue Structure,Trichome and Exudates of Different Flue-cured Tobacco Varieties (Lines)

In order to investigate the difference in leaf tissue structure, trichome development and metabolism characteristics of trichome exudates of different flue-cured tobacco varieties (lines), this paper compared leaf tissue structure, trichome densities and trichome exudates of flue-cured tobacco varieties (lines) including high-aroma 8306 and Yu11 etc., by technology combining scanning electron microscopy, digital microscope and GC-MS. The results showed that: ① The long handle trichomes was predominant trichome type. The quantity of upper glandular trichomes were more than that of lower ones. The densities of short handle trichomes in Yu11, long handle trichomes in 8306, and non-glandular trichomes in K326 were higher than that of the other flue-cured tobacco varieties. ② Cembratrien-diols were main chemical compounds in trichome exudates. Labdanoids including cis-abienol and labdenediol had not been detected in these flue-cured tobacco varieties except Yu11, 8306 and 12451. ③ There was significant positive correlation between radio of palisade tissue to spongy, radio of palisade tissue to leaf thickness, trichome density and trichome exudates. On the whole, the leaf tissue structure, trichome density and trichome exudates of high-aroma flue-cured tobacco varieties (lines) were significantly higher than those of other varieties.