甲基睾酮免疫胶体金试纸条的研制及在水产品中的应用 |
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引用本文: | 夏武强,胡叶军,孙琛,张美珍,王伟萍,桑丽雅. 甲基睾酮免疫胶体金试纸条的研制及在水产品中的应用[J]. 淡水渔业, 2016, 0(1): 93-98. DOI: 10.3969/j.issn.1000-6907.2016.01.015 |
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作者姓名: | 夏武强 胡叶军 孙琛 张美珍 王伟萍 桑丽雅 |
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作者单位: | 1. 象山县海洋与渔业质量技术检测中心,浙江宁波,315700;2. 杭州南开日新生物技术有限公司,杭州,310051 |
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基金项目: | 宁波市水产病害防控与安全科技创新团队(2013B82012) |
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摘 要: | 通过研究胶体金免疫层析技术,建立了一种快速检测水产品中甲基睾酮含量的方法。将待测样品中的甲基睾酮和试纸条检测线上包被的人工抗原(MT-BSA)竞争性结合金标结合垫上包被的胶体金-甲基睾酮单抗聚合物,根据检测线呈现的红色线条深浅来判定样品的阴阳性。结果显示:试纸条上MT-BSA抗原工作浓度为1.0mg/m L,羊抗鼠二抗工作浓度为1.5 mg/m L,试纸条的最低检测限达到10μg/kg,与甲基睾酮类似物的交叉反应率低;使用甲醇提取,4倍PBST稀释,整个检测所需时间仅10~15 min,适用于大规模样品筛选。
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关 键 词: | 甲基睾酮 胶体金 水产品 快速检测 试纸条 |
Development of a coloidal gold immunochromatographic strip for methyltestosterone residue in aquatic products |
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Abstract: | A rapid coloidal gold immunochromatographic assay for methyltestosterone ( MT) residue in aquatic products was developed.The coloidal gold-MT-McAb conjugate coated on the colloidal gold conjugate pad would combine with the MT in the samples and the MT-BSA on the detective line competitively, thus indicated the result of test.The results of this article showed that working concentration of MT-BSA was 1.0 mg/mL and goat anti-mouse IgG was 1.5 mg/mL, the detec-tion limit of the strip could be 10 μg/kg, and the cross-reactivity was low, MT was extracted by methanol, and then the extract was diluted by 4 times PBST solution, the result could be obtained in 10~15 min.Therefore the strip was suitable for the sample screening and qualitative determination. |
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Keywords: | methyltestosterone coloidal gold aquatic products rapid test strip |
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