大豆异黄酮对去卵巢大鼠小脑ERα、NGF、IL-2、AR mRNA表达的影响

建立去卵巢SD大鼠模型,运用原位组织杂交方法,通过补充不同剂量大豆异黄酮观察其对ERα、NGF、IL-2、AR mRNA在小脑中的表达和分布影响,探讨大豆异黄酮对小脑的作用机制和意义及其剂量关系。结果显示:ERα、NGF、IL-2、AR mRNA杂交信号主要分布于小脑皮质的蒲肯野氏细胞层和小脑室顶核、间位核和齿状核中,小脑皮质的分子层和颗粒层也有少量弱杂交信号表达;杂交信号主要定位于胞核,也存在于胞浆、胞膜和突起中,4种杂交信号变化的总趋势为去卵巢大鼠小脑皮质及小脑核中ERα、NGF、IL-2、AR mRNA的表达强度和阳性数目显著降低;而补充不同剂量大豆异黄酮后,4种物质的阳性杂交信号强度和数目均有不同程度回升,其中高剂量组基本恢复到假手术组水平,低剂量组仅有少许回升或无变化,中剂量组介于两者之间。从以上结果得出:大豆异黄酮可在基因水平上促进ERα、NGF、IL-2、AR在小脑中的表达,且存在剂量依赖性;这4种物质表达变化的相似性提示四者在大豆异黄酮对小脑的作用中是相互调节和影响的。

Effects of soybean isoflavone on mRNA expression of ER-alpha,NGF,IL-2 and AR in cerebellum of ovariectomized rat

To observe the direct accommodation and effect of different dosage soybean isoflavone on the cerebellum of ovariectomized rat, the expression and distribution of estrogen receptor alpha (ERα), nerve growth factor (NGF), interleukin-2 (IL-2) and androgen receptor (AR) mRNA in the cerebellum was studied by establishing the model of ovariectomized rat and using in situ hybridization technique. The results indicate that the hybridizational signals of ERα, NGF, IL-2 and AR were mainly distributed in stratum Purkinje cell of the cerebellar cortex, nucleus fastigii, nucleus interpositus and nucleus dentatus of cerebellum, meanwhile their weak signals could also be found in the molecular and granular layer of the cerebellar cortex. In addition, the positive production mostly located in nucleus of the positive cells, and small amounts located in cytoplasm, cytomembrane or dendrite. The general tendency of the expression of ERα, NGF, IL-2 and AR positive production in the cerebellar cortex and nuclei of OVX rat decreased significantly in ovariectomized rats, while the intensity and quantity of the hybridization signals ascended with the supplement of soybean isoflavone. In the OVX group treated with soybean isoflavone of high dosage (OVX+H), the intensity and quantity of these hybridization signals could basically reach the level of those in the sham group. In short, these findings indicated that soybean isoflavone could upregulate the expression of ERα, NGF, IL-2, AR in genic levels with dose-dependent, and the similarity of their changing tendency implied that they were correlated and cooperated during the course of effect of soybean isoflavone on cerebellum.