| 猪输血传播病毒(TTV)与猪圆环病毒混合感染的检测及TTV遗传变异分析 |
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| 引用本文： | 刘建波,郭龙军,危艳武,黄立平,吴洪丽,刘长明.猪输血传播病毒(TTV)与猪圆环病毒混合感染的检测及TTV遗传变异分析[J].中国预防兽医学报,2011,33(1). |
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| 作者姓名： | 刘建波 郭龙军 危艳武 黄立平 吴洪丽 刘长明 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/猪传染病研究室,哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金项目(30871859); 中央级公益性科研院所基本科研业务费(2008-07) |
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| 摘 要： | 为了解我国猪群中猪输血传播病毒(TTV)与猪圆环病毒(PCV)混合感染情况,本研究采用PCR方法对采自14个省280份病料样品进行检测,结果显示,TTV1和TTV2阳性检出率分别为51.8%和28.2%,TTV1与TTV2混合感染率为18.2%;PCV1和PCV2阳性率分别为41.1%和37.5%,PCV1与PCV2混合感染率为19.6%;PCV2阳性样品中TTV1和TTV2混合感染率达75.0%。此外,对2株TTV1基因组进行测序,与GenBank中登录的6个TTV1序列比对,相似性结果为67.3%～95.1%;对4株TTV2基因组测序,与GenBank登录的4条TTV2序列比对,相似性为84.7%～90.4%;将TTV1与TTV2进行序列比对,相似性仅为44.0%。TTV1和TTV2基因高变区分别位于520 nt～2 594 nt和720 nt～2 170 nt;TTV1基因保守区位于1 nt～520 nt和2 595 nt～2 800 nt;而TTV2基因保守区位于1 nt～719 nt和2 171 nt～2 800 nt。以上结果表明,我国猪群中存在TTV与PCV2的混合感染,并且两种病毒混合感染引起...
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| 关 键 词： | 猪输血传播病毒 猪圆环病毒 混合感染 遗传变异分析 |
Detection of co-infection of porcine torque teno virus(TTV)and porcine circovrius and genetic variation analysis for TTV |
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| LIU Jian-bo,GUO Long-jun,WEI Yan-wu,HUANG Li-ping,WU Hong-li,LIU Chang-ming.Detection of co-infection of porcine torque teno virus(TTV)and porcine circovrius and genetic variation analysis for TTV[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(1). |
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| Authors: | LIU Jian-bo GUO Long-jun WEI Yan-wu HUANG Li-ping WU Hong-li LIU Chang-ming |
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| Institution: | LIU Jian-bo,GUO Long-jun,WEI Yan-wu,HUANG Li-ping,WU Hong-li,LIU Chang-ming*(Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | To investigate the co-infection situation of porcine torque teno virus(TTV) and porcine circovrius(PCV),a total of 280 tissue samples from 14 areas was detected by PCR.The results indicated that the positive rate for TTV1 and TTV2 infection were 51.8% and 28.2%,respectively,and the co-infection rate for TTV1 and TTV2 was 18.2%.The positive rate of PCV1 and PCV2 infection was 41.1% and 37.5%,respectively,and co-infection rate of PCV1 and PCV2 was 19.6%.In addition,the co-infection rate of TTV1 and TTV2 reach... |
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| Keywords: | porcine torque teno virus porcine circovrius co-infection analysis of genetic variation |
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