| Ⅰ型鸭肝炎病毒内部核糖体进入位点的结构与功能研究 |
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| 引用本文： | 赵伟,李传峰,陈宗艳,刘光清.Ⅰ型鸭肝炎病毒内部核糖体进入位点的结构与功能研究[J].中国预防兽医学报,2011,33(1). |
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| 作者姓名： | 赵伟 李传峰 陈宗艳 刘光清 |
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| 作者单位： | 1. 中国农业科学院上海兽医研究所,国家家禽工程技术研究中心,上海,200241;西北农林科技大学动物医学院,陕西,杨凌,712100
2. 中国农业科学院上海兽医研究所,国家家禽工程技术研究中心,上海,200241 |
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| 基金项目： | 中央级公益性科研院所专项(2009JB01); 公益性行业(农业)科研专项资助(201003012) |
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| 摘 要： | 为验证Ⅰ型鸭肝炎病毒(DHV-Ⅰ)基因组5'非编码区(UTR)是否含有内部核糖体进入位点元件,本研究利用体外翻译系统,结合细胞转染试验对DHV-Ⅰ5'UTR的结构和功能进行分析。结果表明:DHV-Ⅰ的5'UTR具有典型的内部核糖体结合元件(IRES)功能,能够内部起始下游蛋白的翻译,IRES元件位于360 nt～620 nt,共260个碱基。对IRES的二级结构进行预测和分析显示,DHV-ⅠIRES的结构与丙型肝炎病毒相似。利用定点突变方法,破坏IRES的颈环结构(SL)1、2以及IIIe区的关键性核苷酸,检测该突变对IRES功能的影响。研究结果显示,SL1、SL2和IIIe结构的破坏使得DHV-ⅠIRES的功能消失,表明SL1、SL2和IIIe区是维持IRES启动内部翻译起始功能的关键性结构域。
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| 关 键 词： | Ⅰ型鸭肝炎病毒 5'非编码区 内部核糖体进入位点 |
The structure and function of internal ribosome entry site(IRES) in 5'UTR of duck hepatitis virus type Ⅰ |
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| ZHAO Wei,LI Chuan-feng,CHEN Zong-yan,LIU Guang-qing.The structure and function of internal ribosome entry site(IRES) in 5''UTR of duck hepatitis virus type Ⅰ[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(1). |
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| Authors: | ZHAO Wei LI Chuan-feng CHEN Zong-yan LIU Guang-qing |
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| Institution: | ZHAO Wei1,2,LI Chuan-feng1,CHEN Zong-yan1,LIU Guang-qing1*(1.Shanghai Veterinary Research Institute,Chinese Academy of Agriculatural Sciences,Shanghai 200241,China,2.China College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China) |
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| Abstract: | This paper studied on the relationship between duck hepatitis virus type I(DHV-I) 5' UTR and protein expression.The results showed that DHV-I 5' UTR was a typical internal ribosome entry site(IRES) element,which could initiate the expression of downstream coding region.The secondary structure prediction showed that DHV-I IRES was located at 360 nt to 620 nt,and had similar structure to that of hepatitis virus.To investigate the relation between the structure and the function of IRES,three mutations were int... |
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| Keywords: | duck hepatitis virus trpe 1 5' UTR internal ribosome entry site |
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