蛋白质来源对瘤胃细菌和原虫群体结构的影响

以4只瘘管山羊为试验动物,采用4×4拉丁方设计,使用克隆测序、遗传指纹以及细胞显微计数等技术,研究以羽毛粉(A)、玉米蛋白粉(B)、豆粕(C)和鱼粉(D)4种蛋白补充料分别配合的混合日粮对瘤胃细菌和原虫群体结构的影响。结果表明:细菌的R.flavefaciens、R.bromii、R-7、Roseburia faecalis、Uncultured T33H60F48、Uncultured GRANT42及1个尚不能定性的类群在组间存在显著差异(P<0.05);原虫的内毛虫、前毛虫、头毛虫、等毛虫4个类群在处理间差异显著(P<0.05)。综上可见,蛋白质源对瘤胃细菌和原虫类群结构有显著影响。

Effects of Dietary Protein Sources on Bacterial and Protozoal Community Structure in Goat Rumen

The objectives of this paper were to investigate the effect of protein sources on rumen microbial population,including bacteria and protozoa.Four goats fitted with rumen cannulas,were used in a 4×4 Latin square design.And formula diets were divided into 4 groups according to their nitrogen sources,which was feather meal(A),corn gluten meal(B),soybean meal(C),and fish meal(D),respectively.The cloning and sequence analysis,SSCP fingerprint,and cell-counting techniques were applied in this study.The results showed that significant differences were found in several kinds of bacteria,such as R.flavefaciens,R.bromii,R-7,Roseburia faecalis,Uncultured T33H60F48,Uncultured GRANT42,and an unknown group,and also,there were marked differences in 4 protozoal genuses,such as Entodinium,Epidinium,Ophryoscolex,and Isotrichidae,among protein sources.In conclusion,the protein sources had important effects on ruminal bacterial and protozoal community structure.