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| 籽鹅卵巢组织中铁蛋白重链基因和8个新ESTs的定量研究 | |
| 引用本文： | 康波,姜冬梅,刘本君,杨焕民.籽鹅卵巢组织中铁蛋白重链基因和8个新ESTs的定量研究[J].中国畜牧杂志,2011,47(5). |
| 作者姓名： | 康波 姜冬梅 刘本君 杨焕民 |
| 作者单位： | 1. 四川农业大学动物科技学院,四川雅安,625014 2. 黑龙江畜牧兽医职业学院,黑龙江双城,150111 3. 黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319 |
| 摘 要： | 采用实时定量PCR技术定量检测产蛋前期和产蛋期籽鹅卵巢组织中铁蛋白重链基因(FTH)和8个新ESTs(ODEUG01~ODEUG08)的mRNA表达水平。结果表明:产蛋期籽鹅卵巢组织中FTH和8个新ESTs的相对表达量分别比产蛋前期卵巢组织高13.25、7.68、11.82、14.23、8.25、15.14、9.78、6.48、14.21倍。本研究进一步证实,FTH和8个新ESTs在产蛋期籽鹅卵巢组织中高效表达,FTH和8个新ESTs可能参与籽鹅卵巢功能的调节,并影响籽鹅的产蛋性能。 |
| 关 键 词： | 卵巢 实时定量PCR 表达序列标签 籽鹅 |
Quantitative Studies on Expression Level of FTH and Eight ESTs mRNA in the Ovaries of Zi Geese |
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| KANG Bo,JIANG Dong-mei,LIU Ben-jun,YANG Huan-min.Quantitative Studies on Expression Level of FTH and Eight ESTs mRNA in the Ovaries of Zi Geese[J].Chinese Journal of Animal Science,2011,47(5). | |
| Authors: | KANG Bo JIANG Dong-mei LIU Ben-jun YANG Huan-min |
| Abstract: | Expression level of FTH(ferritin heavy chain) and eight ESTs(ODEUG01 ~ ODEUG08) mRNA of ovaries in prelaying and laying Zi geese were measured by quantitative real-time PCR.The results showed that the expression level of FTH and eight ESTs mRNA in the ovaries of laying geese were increased by 13.25,7.68,11.82,14.23,8.25,15.14,9.78,6.48,14.21 fold,respectively compared to those of prelaying geese.These findings suggested that FTH and eight ESTs are higher in the ovaries of laying geese compared to prelaying ... |
| Keywords: | ovary quantitative real-time PCR expressed sequence tag Zi goose |
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