| 鹅源H5N1亚型禽流感病毒感染雏鸡免疫器官细胞凋亡的研究 |
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| 引用本文： | 张瑞莉,刘明,张卓,郑世民. 鹅源H5N1亚型禽流感病毒感染雏鸡免疫器官细胞凋亡的研究[J]. 中国预防兽医学报, 2011, 33(3). DOI: 10.3969/j.issn.1008-0589.2011.03.03 |
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| 作者姓名： | 张瑞莉 刘明 张卓 郑世民 |
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| 作者单位： | 1. 东北农业大学动物医学学院,黑龙江哈尔滨,150030
2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金项目(30371066) |
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| 摘 要： | 为研究鹅源H5N1亚型禽流感病毒(AIV)人工感染雏鸡免疫器官细胞凋亡的动态变化,本研究将50只1日龄SPF雏鸡随机分为两组。试验组雏鸡于7日龄,分别经鼻、眼、口同时感染105TCID50的鹅源H5N1亚型AIV,分别于感染后3d、4d、5d、7d和14d迫杀,采取胸腺、法氏囊和脾脏,应用TUNEL染色法和透射电镜技术观察其细胞凋亡的动态变化情况。结果显示,试验组雏鸡的胸腺和脾脏凋亡细胞数量在感染后3d～7d极显著高于对照组(p<0.01);法氏囊凋亡细胞数量在感染后3d～4d比对照组明显增加(p<0.05或p<0.01)。组织器官超微结构检测可见凋亡细胞核染色质固缩并凝结成块,聚集在核膜周围,呈新月状或环状;细胞质浓缩。表明鹅源H5N1AIV能够诱导感染雏鸡免疫器官发生细胞凋亡。
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| 关 键 词： | 禽流感病毒 免疫器官 细胞凋亡 |
Apoptosis in the immune organs of chickens infected with H5N1 avian influenza virus derived from goose |
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| ZHANG Rui-li,LIU Ming,ZHANG Zhuo,ZHENG Shi-min. Apoptosis in the immune organs of chickens infected with H5N1 avian influenza virus derived from goose[J]. Chinese Journal of Preventive Veterinary Medicine, 2011, 33(3). DOI: 10.3969/j.issn.1008-0589.2011.03.03 |
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| Authors: | ZHANG Rui-li LIU Ming ZHANG Zhuo ZHENG Shi-min |
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| Affiliation: | ZHANG Rui-li1,LIU Ming2,ZHANG Zhuo2,ZHENG Shi-min1*(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China,2.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | To investigate the apoptosis in the immune organs of chickens infected with H5N1 avian influenza virus(AIV) derived from goose.Fifty one-day-old specific-pathogen-free chickens were divided randomly into two groups,infected group(I group) and control group(C group).I group chickens were inoculated with 0.1 mL(105 TCID50) of H5N1 AIV on 7 days old by nasal,eye and oral route simultaneously.The thymus,bursa and spleen from two groups of chickens were taken at 3,4,5,7 and 14 day post inoculation(dpi),respectiv... |
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| Keywords: | avian influenza virus immune organ apoptosis |
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