| 天津野生白刺再生体系的建立 |
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| 引用本文： | 何斌琼,杨海萍,朱文碧,杨静慧,刘艳军,穆俊丽. 天津野生白刺再生体系的建立[J]. 北方园艺, 2010, 0(10): 159-161 |
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| 作者姓名： | 何斌琼 杨海萍 朱文碧 杨静慧 刘艳军 穆俊丽 |
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| 作者单位： | 西南大学,园艺学院,重庆,400716;天津农学院,园艺系,天津,300384;西北农林科技大学,园艺学院,杨凌,陕西,712100 |
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| 基金项目： | 天津市自然科学基金资助项目，天津市科委科技支撑计划资助项目，天津市农业科技成果转化与推广资助项目 |
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| 摘 要： | 以天津野生白刺为材料,通过不同激素、不同外植体、不同含量的MS培养基和赤霉素的筛选试验建立了白刺再生体系。结果表明:以初春时节白刺幼嫩茎段为外植体建立无菌系,以破坏生长点的茎尖为外植体进行再生;最适芽增殖培养基为MS+BA 3.0 mg/L+NAA 0.4 mg/L,最适增粗培养基为1/2MS+BA 1.0 mg/L+0.2 mg/L IAA,最适芽再生培养基是MS+BA 3.0 mg/L+NAA 0.4 mg/L+GA 3.0 mg/L,再生率为94%。最适伸长培养基为MS+BA 1.0 mg/L+IAA0.3 mg/L,最适生根培养基为1/2MS+IBA 0.5 mg/L。
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| 关 键 词： | 白刺 组织培养 再生 |
Regeneration System of Tianjin Wide N. tangutorum |
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| HE Bin-qiong,YANG Hai-ping,ZHU Wen-bi,YANG Jing-hui,LIU Yang-jun,MU Jun-li. Regeneration System of Tianjin Wide N. tangutorum[J]. Northern Horticulture, 2010, 0(10): 159-161 |
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| Authors: | HE Bin-qiong YANG Hai-ping ZHU Wen-bi YANG Jing-hui LIU Yang-jun MU Jun-li |
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| Affiliation: | HE Bin-qiong1,YANG Hai-ping2,ZHU Wen-bi2,YANG Jing-hui2,LIU Yang-jun2,MU Jun-li3(1.College of Horticulture , L,scape,Southwest University,Chongqing 400716,2.Department of Horticulture,Tianjin Agricultural College,Tianjin 300384,3.College of Horticulture,Northwest Agriculture , Forestry University,Yangling,Shaanxi 712100) |
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| Abstract: | The wild N.tangutorum of Tianjin was selected as the material of this experiment.According to the selection experiments of different concentrations of hormone,different explants,contents of MS culture media and gibberellin,the regeneration system was built up.The results showed that the early spring tender stems were the best explants for the asepsis system,and the damaged caudex tip were used as the explant to regenerate.The best multiplication culture medium was MS+BA 3.0 mg/L+NAA 0.4 mg/L,the best cultur... |
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| Keywords: | N.tangutorum tissue culture regeneration |
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