| 共轭双键对脂肪酸紫外吸收的影响 |
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| 引用本文： | 王冬梅,郭书贤,刘凤霞,薛刚,王莹,冯建波. 共轭双键对脂肪酸紫外吸收的影响[J]. 安徽农业科学, 2007, 35(20): 5999-6000,6002 |
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| 作者姓名： | 王冬梅 郭书贤 刘凤霞 薛刚 王莹 冯建波 |
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| 作者单位： | 南阳理工学院生物与化学工程学院,河南南阳,473004;河南南阳普康药业有限公司,河南南阳,473004 |
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| 基金项目： | 河南省科技攻关项目(0424240033)。 |
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| 摘 要： | [目的]测定共轭亚油酸及部分非共轭脂肪酸的紫外吸收,研究共轭双键对脂肪酸紫外吸收的影响。[方法]测定多种脂肪酸及其混合物的吸光度,并利用气相色谱法测定脂肪酸组分含量。[结果]当加入少量的共轭亚油酸时,混合脂肪酸的紫外吸收曲线表现出明显变化。[结论]共轭双键对脂肪酸在230～240nm范围内的紫外吸收有显著影响,从而为共轭成分的存在提供了检测依据。
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| 关 键 词： | 紫外分光光度法 气相色谱法 亚油酸 共轭亚油酸 γ-亚麻酸 |
| 文章编号： | 0517-6611（2007）20-05999-02 |
| 修稿时间： | 2007-03-27 |
Influence of Conjugated Double Bond on Ultraviolet Absorption of Fatty Acids |
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| WANG Dong-mei et al. Influence of Conjugated Double Bond on Ultraviolet Absorption of Fatty Acids[J]. Journal of Anhui Agricultural Sciences, 2007, 35(20): 5999-6000,6002 |
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| Authors: | WANG Dong-mei et al |
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| Affiliation: | School of Biochemical Engineering, Nanyang Institute of Technology, Nanyang, Henan 473004 |
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| Abstract: | [Objective] The aim of this paper was to measure the ultraviolet absorption value of conjugated linoleic acid and some unconjugated fatty acids as well as to study the influence of conjugated double bond on the ultraviolet absorption of fatty acids. [Method] Ultraviolet absorption values of some fatty acids and their compound were measured by ultraviolet spectrophotometry. And formation of fatty acids was measure by gas chromatographic method. [Result] When slight conjugated linoleic acid was added, there were significant changes on the ultraviolet absorption curve of complex fatty acids within the range from 230 nm to 240 nm. [Conclusion] The ultraviolet absorption value of fatty acids was very significant within the range from 230 nm to 240 nm due to the existence of conjugated double bond, which provided reference to test the existence of conjugated double bond. |
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| Keywords: | Ultraviolet spectrophotometry Gas chromatography Linoleic acid Conjugated linoleic acid γ-linolenic acid |
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