| 毒麦种子内生真菌Gloeotinia temulenta ITS序列分析 |
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| 引用本文： | 梁玮莎,易建平,周国梁,印丽萍,周而勋. 毒麦种子内生真菌Gloeotinia temulenta ITS序列分析[J]. 植物检疫, 2006, 0(Z1) |
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| 作者姓名： | 梁玮莎 易建平 周国梁 印丽萍 周而勋 |
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| 作者单位： | 华南农业大学植物病理学系 上海出入境检验检疫局 华南农业大学植物病理学系 广州 |
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| 基金项目： | 科技部“十五”国家重大科技专项“人畜共患病及植物疫病病原体检测技术”的子课题“影响食品安全的植物病原体检测技术研究”(2001BA804A22)的部分研究内容 |
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| 摘 要： | 从国内采集了毒麦种子进行毒麦内生真菌Gloeotiniatemulenta的分离培养,没有能够成功分离到毒麦内生真菌的菌株,苯胺蓝染色镜检观察毒麦种子内的内生真菌带菌率为0~65.4%。直接从毒麦种子中挑取内生真菌菌丝,用基因释放剂(genereleaser,BioVenture)处理后扩增测序获得了毒麦内生真菌的ITS序列(DQ235697),经过相似性比对和分析,所得序列和GenBank中的内生真菌的序列同源性高达99.4%~100%,排除了毒麦种子表面真菌和毒麦种子序列的可能性。
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| 关 键 词： | 毒麦 内生真菌 ITS序列 |
ITS sequence analysis of Gloeotinia temulenta from the seed of Lolium temulentum |
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| Liang Weisha,Yi Jianping,Zhou Guoliang,Yin Liping,Zhou Erxun. ITS sequence analysis of Gloeotinia temulenta from the seed of Lolium temulentum[J]. Plant Quarantine, 2006, 0(Z1) |
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| Authors: | Liang Weisha Yi Jianping Zhou Guoliang Yin Liping Zhou Erxun |
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| Affiliation: | Liang Weisha~1,Yi Jianping~2,Zhou Guoliang~2,Yin Liping~2,Zhou Erxun~1 |
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| Abstract: | The average percentage of endophytic fungus Gloeotinia temulenta in the seeds of Lolium temulentum were 0~65.4%. The test for isolating endophytic fungus from the seeds of L. temulentum was not successful to get the culture of G. temulenta. The DNA of G. temulenta treated with genereleaser (BioVenture) was amplified by universal primers ITS5/ITS4 and sequenced. The ITS sequence (DQ235697) of G. temulenta was obtained and analysed, The similarity of ITS sequence between G. temulenta and other endophytic fungi including Paecilomyces fumosoroseus (AB086629) and Fungal endophyte (AY452984) was more than 99%. |
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| Keywords: | Lolium temulentum Gloeotinia temulenta ITS sequence |
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