| 棉花子叶节芽分化培养基的优化研究 |
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| 引用本文： | 胡根海,王清连,张志勇,李文静,李成奇,张金宝. 棉花子叶节芽分化培养基的优化研究[J]. 种子, 2011, 30(4) |
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| 作者姓名： | 胡根海 王清连 张志勇 李文静 李成奇 张金宝 |
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| 作者单位： | 河南科技学院生命科技学院,河南,新乡,453003 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 以陆地棉百棉1号为材料,离体培养子叶节,对芽分化培养基中6-BA和KT的使用浓度进行了优化;6-BA和KT浓度各设置(0.5、1.0、2.0、2.5、3.0、5.0 mg/L)5个梯度,比较了单独使用6-BA、KT和2种激素组合时对诱导多芽发生的差异;结果显示,单独使用6-BA对诱导芽分化效果较好,单独使用KT可以在芽分化的同时,诱导根的生成.组合使用两种激素时,效果优于单独使用;组合使用1.0mg/L6-BA+2.0mg/L KT时效果最佳.
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| 关 键 词： | 百棉1号 子叶节 丛生芽 丛生芽诱导 |
Study on Optimization Bud Differentiation Medium of Colyledon Nodes in Upland Cotton |
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| HU Gen-hai,WANG Qing-lian,ZHANG Zhi-yong,LI Wen-jing,LI Cheng-qi,ZHANG Jin-bao. Study on Optimization Bud Differentiation Medium of Colyledon Nodes in Upland Cotton[J]. Seed, 2011, 30(4) |
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| Authors: | HU Gen-hai WANG Qing-lian ZHANG Zhi-yong LI Wen-jing LI Cheng-qi ZHANG Jin-bao |
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| Affiliation: | HU Gen-hai,WANG Qing-lian,ZHANG Zhi-yong,LI Wen-jing,LI Cheng-qi,ZHANG Jin-bao(School of Life Science and Technology,He'nan Institute of Science and Technology,Xinxiang,He'nan 453003,China) |
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| Abstract: | Upland cotton Baimian No.1 was used as the test material,and cultured section of the cotyledon in vitro.Experimental objectives was to find the best combination of shoot differentiation medium.6-BA and KT hormones were set 5 various concentration gradients(0.5,1.0,2.0,2.5,3.0,5.0mg/L),the difference on the induction of multiple shoots were compared by alone 6-BA,KT and two hormone combinations.The result showed that the 6-BA alone played a key role on inducing buds differentiation,and KT alone not only coul... |
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| Keywords: | baimian No.1 cotyledonary node multiple buds multiple shoot induction |
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