| 金属离子对纤维素酶活性影响的研究 |
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| 引用本文： | 程旺开. 金属离子对纤维素酶活性影响的研究[J]. 安徽农学通报, 2011, 17(5): 27-28,30 |
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| 作者姓名： | 程旺开 |
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| 作者单位： | 芜湖职业技术学院生物工程系,安徽芜湖,241000 |
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| 基金项目： | 芜湖职业技术学院自然科学研究项目 |
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| 摘 要： | 通过金属离子对纤维素酶系中3大组分（β-葡萄糖苷酶、内切葡聚糖酶和外切葡聚糖酶）酶活的影响，探讨金属离子对纤维素酶活影响的作用机理。试验结果表明，金属离子Co2＋、Zn2＋、Mn2＋对内切葡聚糖酶酶活具有促进作用；Co2＋和K＋对外切葡聚糖酶的酶活具有促进作用；Co2＋、Mn2＋、Ca2＋和Mg2＋对β-葡萄糖苷酶具有促进作用，来源不同的纤维素酶，因其酶系组分不一样，酶促离子对纤维素酶的促进作用有所差异。
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| 关 键 词： | 金属离子 纤维素酶活 β-葡萄糖苷酶 内切葡聚糖酶 外切葡聚糖酶 |
Effect of Metal Ions on the Activity of Cellulase |
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| Cheng Wangkai. Effect of Metal Ions on the Activity of Cellulase[J]. Auhui Agricultural Science Bulletin, 2011, 17(5): 27-28,30 |
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| Authors: | Cheng Wangkai |
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| Affiliation: | Cheng Wangkai ( Department of Biochemical, Wuhu Institute of Technology, Wuhu 241000, China) |
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| Abstract: | The study of the three major components （ β- glucosidase, endo - 1,4 - [3 - D - glucanase and exo - 1,4 - β-D -glucanase） enzyme activity was influenced by the metal ions. The results showed that Co2＋ , Zn2 ＋ , Mn2＋ were promoted to internal endoglucanase activity, Co2 ＋ and K ＋ were promoted to External endoglucanase activity, Co2 ＋ , Mn2 ＋ , Ca2 ＋ and Mg2 ＋ were enhanced the β- glucosidase activity. Because of its different component, the activity of enzymes cellulose from different sources was found to be dissimilar under the same metal ions concentration. |
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| Keywords: | Metal ions Cellulase activity β-glucosidase Endo - 1,4 -β-D -glucanase Exo - 1,4 - β- D -glucanase |
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