| 高粱DNA导入小麦"矮早8"引起后代变异的研究及SSR分析 |
| |
| 引用本文： | 张希太.高粱DNA导入小麦"矮早8"引起后代变异的研究及SSR分析[J].安徽农学通报,2011,17(5):41-43,57. |
| |
| 作者姓名： | 张希太 |
| |
| 作者单位： | 邯郸市农业科学院生物技术中心,河北邯郸,056001 |
| |
| 摘 要： | 将高梁“抗5”DNA通过花粉管通道导入小麦品种“矮早8”，其后代在株高、蜡质层、芒型、穗型、旗叶、成熟期等几个方面发生大量变异。通过对变异后代系统选择得到多个稳定的变异种质系；通过对“矮早8”、D8—1、D8—4、高粱“抗5”基因组DNA的SSR分子标记检测，引物xgwm6、xgwm148、xgwm295、xgwm328、xgwm257检测出了较丰富的多态性DNA片段．其中xgwm6、xgwm257检出了高粱“抗5”DNA特有而“矮早8”没有的特异带型，进一步说明了D8—1、D8—4是由高粱“抗5”DNA片段嵌入“矮早8”基因组DNA变异而来。
|
| 关 键 词： | 高粱DNA 花粉管通道 矮早8 后代变异 SSR分子标记 |
Study on Character Variation Induced by Broomcorn DNA Intrducing into the Wheat "Aizao8" and Assessment of Genetic Diversity of the Descendant by SSR Markers |
| |
| Zhang Xitai.Study on Character Variation Induced by Broomcorn DNA Intrducing into the Wheat "Aizao8" and Assessment of Genetic Diversity of the Descendant by SSR Markers[J].Auhui Agricultural Science Bulletin,2011,17(5):41-43,57. |
| |
| Authors: | Zhang Xitai |
| |
| Institution: | Zhang Xitai (Biotechnology Center of Handan Agrocniture Research institute , Handan 056001, China) |
| |
| Abstract: | There are many descendants had been mutated in plant height.waxiness .awn .Ear type.Flag leave .maturation by importing broomcorn DNA of ＂kang5＂ into the wheat ＂Aizao8＂ through the Pollen- tube Pathway~ We have own many unaltered germplasm line by Subsequent continuous selection in the variational descendants ; Detect the DNA of＂ aizao8＂ D8 - 1 .D8 -4.broomcorn＂ kang5＂ Using SSR markers, There are many differential fragments have been found by the Prime xgwm6 ,xgwm148 、 xgwm295, xgwm328, xgwm257, Especially xgwm6, xxgwm257, they have been found the differential fragments in the DNA of D8 - 1 and D8 - 4 that belong to the hroomcorn DNA of ＂kangS＂ only, It further attest that D8 - 1 and D8 -4 was come from ＂AizaoS＇＂ that was inlaid the fragments of broomcorn DNA of ＂kang5 ＂. |
| |
| Keywords: | Broomcorn DNA Pollen - tube Pathway Aizao8 Variational descendants SSR markers |
| 本文献已被 维普 万方数据 等数据库收录！ |
