| 山核桃ISSR反应体系的优化 |
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| 引用本文： | 吕岩桢,王雷,张兴旺,郭传友. 山核桃ISSR反应体系的优化[J]. 安徽农学通报, 2011, 17(7): 30-31,35 |
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| 作者姓名： | 吕岩桢 王雷 张兴旺 郭传友 |
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| 作者单位： | 1. 淮北师范大学生命科学学院,安徽淮北,235000
2. 资源植物生物学安徽省重点实验室,安徽淮北,235000 |
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| 基金项目： | 安徽省教育厅自然科学研究重点项目，国家自然科学基金项目 |
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| 摘 要： | 采用改良的CTAB法提取山核桃基因组DNA,该方法提取的DNA纯度与含量较高。以宁国山核桃居群为试材,用引物UBC810[序列为（GA）8T]研究了PCR反应体系的5种主要组分对山核桃ISSR扩增结果的影响。结果显示：除模板DNA含量外,其它因素均对扩增效果有明显影响。建立的反应体系为：20μL反应体系中DNA模板量30ng、引物浓度0.3μmol/L、dNTPs浓度0.3mmol/L、Mg2＋浓度2.0mmol/L、TaqDNA聚合酶用量1U。
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| 关 键 词： | 山核桃 ISSR 反应体系 优化 |
Optimization of ISSR Reaction system in Carya cathayensis |
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| Lv Yanzhen et al.. Optimization of ISSR Reaction system in Carya cathayensis[J]. Auhui Agricultural Science Bulletin, 2011, 17(7): 30-31,35 |
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| Authors: | Lv Yanzhen et al. |
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| Affiliation: | Lv Yanzhen et al.(School of Life Science,Huaibei Normal University,Huaibei 235000,China,Anhui Key Laboratory of Plant Resources and Biology,China) |
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| Abstract: | DNA was extracted from the young leaves of Carya cathayensis by improved CTAB method.The purity and yield of DNA that extracted by this method was high.The effects of the concentrations of five important PCR reaction components on inter-simple sequence repeat(ISSR) amplification results of Carya cathayensis in Ningguo county were studied with the primer U810[its sequence :(GA)8T].The results showed that the five components but template DNA obviously affected ISSR amplification effects.The optimal concentrat... |
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| Keywords: | ISSR |
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