| RT-PCR和实时荧光RT-PCR一步法检测大豆中菜豆荚斑驳病毒 |
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| 引用本文： | 廖芳,郭京泽,刘鹏,张裕君,黄国明. RT-PCR和实时荧光RT-PCR一步法检测大豆中菜豆荚斑驳病毒[J]. 植物保护学报, 2009, 36(2): 141-145 |
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| 作者姓名： | 廖芳 郭京泽 刘鹏 张裕君 黄国明 |
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| 作者单位： | 廖芳,Liao Fang(天津出入境检验检疫局,天津,300456;南开大学生命科学学院,天津,300071);郭京泽,刘鹏,张裕君,黄国明,Guo Jingze,Liu Peng,Zhang Yujun,Huang Guoming(天津出入境检验检疫局,天津,300456) |
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| 基金项目： | 科技部质检公益性行业科研专项基金 |
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| 摘 要： | 针对进口大豆的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)检测,建立了一步法RT-PCR和一步法实时荧光RT-PCR检测方法.依据BPMV的外壳蛋白编码基因设计了特异引物和特异Taqman探针,特异引物的扩增片段约为500bp,阳性质粒的实时荧光PCR方法的检测下限为20fg/μL,是一步法RT-PCR方法的100倍,检测时间由约8 h缩短至4 h.种脐灰色斑驳的大豆种子BPMV检测呈阳性,种脐黑色斑驳的大豆种子BPMV检测呈阴性.
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| 关 键 词： | 大豆 菜豆荚斑驳病毒 一步法实时荧光RT-PCR 一步法RT-PCR 检测 |
| 收稿时间： | 2008-09-03 |
Detection of Bean pod mottle virus in soybean by one step assay of RT-PCR and real-time fluorescent RT-PCR |
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| Liao Fang,Guo Jingze,Liu Peng,Zhang Yujun and Huang Guoming. Detection of Bean pod mottle virus in soybean by one step assay of RT-PCR and real-time fluorescent RT-PCR[J]. Acta Phytophylacica Sinica, 2009, 36(2): 141-145 |
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| Authors: | Liao Fang Guo Jingze Liu Peng Zhang Yujun Huang Guoming |
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| Affiliation: | Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300456, China; College of Life Sciences, Nankai University, Tianjin 300071, China;Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300456, China;Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300456, China;Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300456, China;Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300456, China |
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| Abstract: | |
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| Keywords: | soybean Bean pod mottle virus one step real-time fluorescent RT-PCR one step RT-PCR detection |
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