补铁对实验性肠炎仔猪肠道结构、功能及体内抗氧化酶活性的影响

利用右旋糖苷硫酸钠(DSS)诱导仔猪肠炎模型,探讨口服铁对患肠炎的仔猪肠道功能和氧化应激参数的影响。试验选取50头21日龄早期断奶仔猪,按体重、性别随机分为5组,每组10头。对照组饲喂基础日粮,饮用自来水,另外4组分别饲喂添加0、0.03%、0.20%和1.00%FeSO4的日粮,饮用含4%DSS的水。结果表明:随着口服铁剂量的增加,肠道微绒毛的损伤逐渐加重。与DSS单独处理组相比,高剂量的铁显著提高了尿中乳果糖/甘露醇的排泄比率,显著降低了血浆中D-木糖的浓度(P<0.05),而低剂量的铁对其没有显著影响。铁的添加显著降低了过氧化氢酶(CAT)和谷胱甘肽过氧化酶(GPx)的活性(P<0.05),而对超氧化物歧化酶(SOD)活性影响不明显。

Effect of iron supplementation on intestinal structure,function and antioxidation enzyme activity of the piglets in experimental colitis

To assess the effect of different doses of iron on the intestinal function and oxidative stress in piglets with dextran sulphate sodium(DSS)-induced colitis,fifty 21-day-old weaned piglets were randomly divided into five groups of ten each.The piglets received regular diet and water,or four diets with 0,0.03,0.20 or 1.00% FeSO4 and water with 4% DSS respectively.We observed a markedly higher lactulose/mannitol excretion ratio and lower serum concentration of D-xylose in FeSO4 0.20% and 1.00% supplementation piglets than piglets on DSS alone,but not in FeSO4 0.03% supplementation piglets.Supplementation of oral iron resulted in intestinal villus injury in piglets,and dose-response manner.The activities of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GPx) significantly increased in the DSS group compared with the control group.Iron supplementation significantly decreased the activities of GPx and CAT(P<0.05),but not SOD.