应用高效液相色谱-蒸发光散射检测法测定大蒜中糖含量

目的]建立简便、准确、灵敏的测定大蒜中果糖、葡萄糖及蔗糖含量的方法。方法]样品经热水提取后,使用Kromasil NH2(250mm×4.6 mm,5μm)柱分离,以乙腈-水(80∶20,V/V)为流动相,使用蒸发光散射检测器检测。结果]果糖、葡萄糖和蔗糖的线性范围分别为0.05~3、0.075~5、0.05~5 g/L;检出限分别为1.9、2.4、1.8μg/L;样品的平均加标回收率分别为99.2%、97.6%、98.0%;相对标准偏差分别为1.02%、1.41%、1.27%。结论]该方法操作简便、快速、准确,用于实际样品的测定,结果令人满意。

Determination of Carbohydrates in Allium sativum L. By High Performance Liquid Chromatography with Evaporative Light Scattering Detection

Objective] The aim was to develop a simple,accurate and sensitive method for determination of carbohydrates in Allium sativum L.Method] The carbohydrates in Allium sativum L.were extracted with hot water and separated on Kromasil NH2 column(250 mm×4.6 mm,5 μm) using acetonitrile and water as the mobile phase with volume ratio of 80∶20.The elute from the column was examined by an evaporative light scattering detector.Result] The linear ranges of fructose,glucose and sucrose were 0.05-3,0.075-5 and 0.05-5 g/L.The detection limits were 1.9,2.4,and 1.8 μg/L.The average recovery were 99.2%,97.6% and 98.0%,and the relative standard deviation were 1.02%,1.41% and 1.27%,respectively.Conclusion] The method was applied to the determination of carbohydrates in Allium sativum L.with satisfactory results and it was found to be simple,fast and accurate.