接种平滑白蛋巢菌对蒿柳根系分泌物代谢组的影响

目的 虽然已有研究表明植物-白腐真菌联合修复是一种更高效的多环芳烃（PAHs）污染土壤修复策略，但目前尚不清楚接种白腐真菌对植物根系分泌物的影响，后者与根际PAHs降解菌的生长和PAHs的生物降解密切相关。基于此，我们开展PAHs污染土壤中接种白腐真菌对植物根际土壤代谢组的影响研究。 方法 在温室中设置了PAHs污染土壤的盆栽修复实验，以蒿柳（Salix viminalis L.）作为植物修复树种，基于LC-MS检测，重点比较PAHs污染土壤中接种平滑白蛋巢菌（Crucibulum laeve）对蒿柳根际土壤代谢谱的影响。 结果 分别在正离子模式（POS）和（NEG）模式下鉴定出881和828种代谢物，不同处理之间代谢物的组分及其含量存在显著差异。比较分析了差异代谢物并鉴定出18个潜在根系分泌物组分，接种平滑白蛋巢菌显著减少了蒿柳根际土壤代谢物的组分和含量，其中16种根系分泌物的含量明显降低。 结论 接种白腐真菌促进了植物根系对广谱土壤化合物的吸收能力，推测植物根系对白腐真菌的刺激作用做出应答，引发了植物PAHs提取能力提高，对揭示植物-白腐真菌联合修复的作用机理有重要意义。

Effects of Crucibulum laeve Inoculation on Metabolome in Root Exudate from Salix viminalis L.

Objective To study the effects of plant/white rot fungi (WRF) inoculation on rhizosphere soil metabolome in root exudate of plants cultivated in PAH-contaminated soil. Method A pot experiment was conducted in greenhouse for bioremediation of PAH-contaminated soils and Salix viminalis L. was used as phytoremediation materials. Based on liquid chromatography-mass spectrometry (LC-MS) and metabolomics method, the test was focused on comparing the effect of inoculating Crucibulum laeve on metabolic profiling of rhizosphere PAH-contaminated soil of S. viminalis. Result Under POS and NEG mode, 881 and 823 compounds were detected and identified in metabolic profiling, respectively. Among them, 18 compounds were identified and assumed to be potential root exudates components. The component variety and content of rhizosphere metabolites were remarkably reduced by C. laeve inoculating, which including 16 root exudate components. Conclusion In this study, it is showed that inoculation with WRF can promote the uptake capability of roots to a broad spectrum of soil compounds. It is speculated that the response of plant roots to WRF stimulating leads to the improvement of PAHs phytoextraction capacity. This is of great significance for revealing the mechanism of plant/WRF combination remediation of PAH-contaminated soils.