内蒙古苜蓿锈菌越冬的分子定量检测

苜蓿是世界上种植面积最大的豆科牧草,内蒙古的苜蓿商品草种植面积和产量均居全国第二.但近年来,苜蓿锈病发生日趋严重,很大程度上影响着内蒙古苜蓿产业的发展.2018年—2020年,笔者建立了基于RNA水平的real-time quantitative PCR(qPCR)技术体系,对内蒙古苜蓿主产区乌兰察布市、呼和浩特市、包...

Molecular Quantitative Detection for Overwintering of Uromyces striatus in Inner Mongolia

Alfalfa is a legume forage and is the most widely grown forage crop in the world,the planting area and yield of commercial alfalfa forage in Inner Mongolia rank the second in China.In recent years, the occurrence of alfalfa rust is becoming more and more serious,the development of alfalfa industry in Inner Mongolia is greatly affected.From 2018 to 2020, the real-time quantitative PCR(qPCR) technology was established based on RNA level to detect the overwintering inoculum quantity of Uromyces striatus in Ulanqab, Hohhot, Baotou, Ordos and Bayannuthe, which are the main alfalfa producing areas in Inner Mongolia.The results showed that the mean molecular disease index(MDI) of overwintering U.striatus was 0.139, 0.284, 0.196, 0.569 and 0.280, respectively.The mean MDI of Ordos city was significantly higher than that of the other four areas (P<0.001).The mean MDI of overwintering U.striatus was 0.43 in 2019, which was significantly higher than that of the other years in 2018(0.24, P<0.05) and in 2020(0.193, P<0.05).U.striatus could overwinter underground rhizomatous tissue of alfalfa.There was a positive correlation between the amounts of overwintering U.striatus and the disease incidence of alfalfa rust after plants regreening (r=0.806, P<0.001).