荷叶中槲皮素提取工艺研究

[目的]优化荷叶提取槲皮素的工艺。[方法]研究了3种提取方法对槲皮素转移率的影响;通过对荷叶中槲皮素的回流提取条件进行4因素3水平正交试验,确定了荷叶中槲皮素的最佳提取工艺。[结果]采用乙醇回流提取法提取荷叶中槲皮素效果最佳,槲皮素转移率均高于60%,明显优于乙醇温浸法(<16.2%)和碱温浸法(<20.0%)。4因素对于槲皮素转移率的影响程度依次为固液比>溶剂浓度>提取时间>提取温度。荷叶中槲皮素最佳提取工艺条件为:乙醇回流提取,乙醇溶液浓度60%,提取温度100℃,料液比12:0,提取时间1 h。[结论]经试验验证,该研究筛选优化的工艺条件基本稳定、可行。在此工艺条件下,槲皮素的转移率在70%以上。

Study on Extraction Technique of Quercetin from Lotus Leaf

[Objective] This study aimed to optimize extraction technique of quercetin from Lotus leaf.[Method] The effects of 3 kinds of extraction methods on transfer rate of quercetin were investigated.Through the L9(34) orthogonal design experiment about conditions of reflux extraction,the optimum extraction process of quercetin from lotos leaf was ascertained.[Result] The ethanol refluxing method of extracting quercetin from L.leaf could get optimum effect,by which the transfer rate of quercetin was more than 60%,being obviously higher than that by ethanol warm immersion(<16.2%) and alkali warm immersion(<20.0%),resp.The influencing degree of 4 factors on transfer rate in order was ratio of solid to liquid>solvent density>extraction time > extraction temperature.The optimum process conditions for extracting quercetin from L.leaf were the ethanol refluxing txtraction,60% ethanol,extraction temperature of 100 ℃,solid-liquid ratio of 1:20,extraction time of 1 h.[Conclusion] The extraction technique screened by this study was stable and feasible on the whole,under which,the transfer rate of quercetin was over 70%.