牛瑟氏泰勒虫套式PCR检测方法的建立及初步应用

根据GenBank上发表的牛瑟氏泰勒虫P33基因序列设计2对特异性引物,建立套式PCR检测方法。该方法与弓形虫RH株、猪附红细胞体和犬新孢子虫均无交叉反应,能检测到的最低DNA量为15 fg。在75份临床样本检测中,62份为阳性,阳性率为82.7%。由此可见所建立的套式PCR检测方法具有较高的敏感性和特异性,可用于牛瑟氏泰勒虫急性感染和隐性感染的早期诊断,为该病的临床检测、流行病学调查、进出口检疫和实验室研究提供了新的技术手段。

Establishment and preliminary application of a nested PCR for the detection of Theileria sergenti

According to the published Theileria sergenti P33 gene sequence in GenBank,two pairs of specific primers were designed to establish a nested PCR detection method.This method had no cross-reaction with Toxoplasma,Eperythrozoon suis and Neospora.The lowest detection amount of DNA could reach 15 fg.The detection results for 75 clinical samples showed 62 were positive and the positive rate was up to 82.7%.The results indicated that the nested PCR method was highly sensitive and specific,and could be used for the early diagnosis of acute and latent infections of T.sergenti.This study provides a new way for clinical detection,epidemiological investigation,import and export quarantine and laboratory studies on T.sergenti.