中国野生葡萄副梢长度的RAPD标记

为培育短副梢葡萄品种,对中国野生葡萄副梢长度性状进行调查,以葡萄短梢×长梢的葡萄种间杂交组合(燕山-1×河岸葡萄)F183个单株为试材,采用BSA法和RAPD技术,通过369个随机引物的筛选,获得了1个与中国野生葡萄副梢长度基因连锁的RAPD标记OPB07-2000,并在中国野生葡萄17个种46个株系中得到验证。这就为葡萄短副梢分子辅助育种提供了科学依据,进而从根本上解决葡萄生产上夏季副梢生长过旺的问题。

RAPD markers of axillary shoot length of Chinese wild grapes

Based on the survey of the axillary shoot length characteristics of the Chinese wild grape species collected and planted at the germplasm resources collection plot in Northwest A & F University, 83 seedlings of the inter-specific F1 hybrid between short length axillary shoot × long length axillary shoot (Yanshan-1 × V. riparia) were examined by employing the methods of random amplified polymorphic DNA (RAPD) markers and bulked segregate analysis (BSA). OPB07-2000, a marker linked to the length of grape axillary shoot, was obtained through a selection among 369 random primers. The marker was verified in Chinese wild grapes. This work may lay a scientific basis for molecular marker-assisted selection (MAS) to choose grape cultivars with short axillary shoot, which will completely settle the problems resulting from the management of grape axillary shoots in summer.