| 利用聚类分析筛选玉米杂交种SSR指纹库中鉴别能力强的标记 |
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| 引用本文： | 王伟,杨文鹏,冯浪,滕安平. 利用聚类分析筛选玉米杂交种SSR指纹库中鉴别能力强的标记[J]. 种子, 2010, 29(10) |
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| 作者姓名： | 王伟 杨文鹏 冯浪 滕安平 |
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| 作者单位： | 贵州省旱粮研究所;贵州省农业生物技术重点实验室;贵州省种子管理站; |
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| 基金项目： | 贵州省科技计划项目，国家科技支撑计划项目 |
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| 摘 要： | 作物DNA指纹库构建后,如何有效应用是亟待解决的问题.本研究首先设定品种间某(些)分子标记位点遗传距离大于零者为某(些)分子标记可鉴别的品种,分析DNA分子标记鉴别品种的能力;在此基础上,设定品种间某(些)分子标记位点最小遗传距离大者为优,筛选出鉴定参试品种所需的最少标记数.然后利用聚美分析,从20个SSR标记构建的48个玉米杂交种的指纹库中,可以筛选出鉴别能力最强的标记和鉴据别效果最优的标记组合.结果表明,该方法直观、易行、快速、高效,可为指纹图谱库的高效应用提供参考.
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| 关 键 词： | 指纹图谱 聚类分析 SSR 玉米 |
Applying Cluster Analysis to Screen SSR Markers with High Identifying Ability from SSR Fingerprint Data of Maize Hybrids |
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| WANG Wei,YANG Wen-peng,FENG Lan,TENG An-ping. Applying Cluster Analysis to Screen SSR Markers with High Identifying Ability from SSR Fingerprint Data of Maize Hybrids[J]. Seed, 2010, 29(10) |
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| Authors: | WANG Wei YANG Wen-peng FENG Lan TENG An-ping |
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| Affiliation: | WANG Wei1,YANG Wen-peng1,2,FENG Lang3,TENG An-ping3(1.Guizhou Institute of Upland Food Crops,Guiyang 550006,China,2.Guizhou Key Laboratory of Agricultural Biotechnology,3.Seed management department of Guizhou,Guiyang,550001,China) |
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| Abstract: | How to effectively use crop DNA fingerprint to identify crop varieties is urgently needed to be solved after establishing fingerprint data.In this study,supposed genetic distance between varieties at a(some)molecular marker locus(loci)was bigger than 0,then considered the molecular marker(s)could identify the varieties and with high identifying ability.Further,supposed least genetic distance between varieties at a(some)molecular marker locus(loci)was maximal,then considered the molecular marker(s)could iden... |
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| Keywords: | fingerprint cluster analysis SSR maize |
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