家蚕质型多角体病毒苏州核包涵体小种的纯化

从BmCPV_t内发现和分离出一株新的核包涵体小种,初步命名为家蚕质型多角体病毒—苏州核包涵体小种(Cytoplasmic Polyhedrosis Virus——Suzhou nuclear polyhedra forming strain,简称CPV——Suzhou N strain),家蚕感梁CPV——Suzhou N Strain引起的多角体病,与国内外已知的CPV各变异株不同。本病蚕的多角体只在中肠组织圆筒形细胞核内检出,多角体内有许多病毒粒子,病毒粒子球状,具有核蛋白紫外吸收特征性光谱和较强的感染活性,病毒核酸初步鉴定为dsRNA。

Purification of cytoplasmic polyhedrosis virus----Suzhou nuclear polyhedra forming strain of silkworm ( Bombyx mori). Sci.Seric.,

A new nuclear polyhedra forming strain has been discovered and isolated from BmCPVt which was tentatively named cytoplasmic polyhedrosis virus--Suzhou nuclear polyhedra forming strain of silkworm, and was called "CPV--Suzhou N strain" for short. The silkworm's polyhedrosis caused by CPV--Suzhou N strain was different from that caused by other known various varieties of CPV. The polyhedra was detected only in the cylindrical cells of midgut of diseased silkworm. Many virus particles were found in the polyhedra. The shapes of the virions were spherical. The viruses have been demonstrated to have the nucleoprotein characteristic UV-absorp-tion spectrum and higher infectivity. The nuclear acid of the virus was identified as dsRNA preliminarily.