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| 藏猪生长激素基因(pGH)多态性研究 | |
| 引用本文： | 张浩,强巴央宗,张明明,彭俊飞,杨涛,达娃卓玛,吴常信.藏猪生长激素基因(pGH)多态性研究[J].西北农业学报,2010,19(2). |
| 作者姓名： | 张浩 强巴央宗 张明明 彭俊飞 杨涛 达娃卓玛 吴常信 |
| 作者单位： | 1. 中国农业大学,动物科技学院,北京100094 2. 西藏农牧学院,动物科学系,西藏林芝860000 3. 安徽省农业科学院,情报研究所,合肥230031 |
| 基金项目： | 国家科技支撑项目，西藏自治区科技计划项目 |
| 摘 要： | 猪生长激素基因(pGH)是猪生长发育性状重要的候选基因之一。采用PCR-RFLP技术检测藏猪pGH基因+159~+734 bp片段的DraI、ApaI和MspI酶切多态性。结果显示,藏猪群体中DraI酶切全部切开,无多态;ApaI酶切具有4个等位基因,出现5种基因型,其中CC基因型(299A/432C)频率最高,为0.7857;藏猪群体MspI酶切出现3种基因型,杂合性CT基因型频率最高,为0.5089,等位基因C和T频率分别为0.5580和0.4420。经比较,藏猪pGH基因DraI酶切位点与其他猪种一样无多态性,而ApaI酶切出现了特异的432突变位点,MspI酶切等位基因分布相对均衡,表现出与其他猪种的差异。 |
| 关 键 词： | 藏猪 pGH基因 多态性 |
Polymorphisms of the Porcine Growth Hormone Gene (pGH) in Tibet Pig |
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| ZHANG Hao,CHAMBA Yangzom,ZHANG Mingming,PENG Junfei,YANG Tao,DAWA Zhuoma,WU Changxin.Polymorphisms of the Porcine Growth Hormone Gene (pGH) in Tibet Pig[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(2). | |
| Authors: | ZHANG Hao CHAMBA Yangzom ZHANG Mingming PENG Junfei YANG Tao DAWA Zhuoma WU Changxin |
| Institution: | ZHANG Hao1,CHAMBA Yangzom2,ZHANG Mingming3,PENG Junfei1,YANG Tao2,DAWA Zhuoma2 , WU Changxin1(1.College of Animal Science , Technology,China Agricultural University,Beijing 100094,China,2.College of Agriculture , Animal Husb,ry,Tibet University,Linzhi Tibet 860000,3.Institute of Information,Anhui Academy of Agricultrul Sciences,Hefei 230031,China) |
| Abstract: | The porcine growth hormone (pGH) gene is one of important candidate for development and growth trait. In the present study, the polymorphism of fragment +159～+734 of pGH gene was measured with the methods of PCR-RFLP by restriction endonuclease Dra I, Apa I and Msp I. The results showed that the Tibet pig has no diversity for PCR-Dra I-RFLP of pGH gene. There are four alleles and five genotypes for PCR-Apa I-RFLP of pGH gene, and the genotype CC (299A/432C) is predominant (0.7857). The Tibet pig population has three genotypes for PCR-Msp I-RFLP of pGH gene, and the predominant genotype CT is (0.5089). The frequencies of allele C and allele T are 0.5580 and 0.4420 respectively. By comparing with other breeds, the Tibet pig has differential genotypes for Apa I, and the distribution of alleles for Msp I is uniform relatively, which may be the difference of Tibet pig with other pig breeds. |
| Keywords: | PCR-RFLP Tibet pig pGH gene PCR-RFLP Polymorphism |
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