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藏猪生长激素基因(pGH)多态性研究
引用本文:张浩,强巴央宗,张明明,彭俊飞,杨涛,达娃卓玛,吴常信. 藏猪生长激素基因(pGH)多态性研究[J]. 西北农业学报, 2010, 19(2)
作者姓名:张浩  强巴央宗  张明明  彭俊飞  杨涛  达娃卓玛  吴常信
作者单位:1. 中国农业大学,动物科技学院,北京100094
2. 西藏农牧学院,动物科学系,西藏林芝860000
3. 安徽省农业科学院,情报研究所,合肥230031
基金项目:国家科技支撑项目,西藏自治区科技计划项目 
摘    要:猪生长激素基因(pGH)是猪生长发育性状重要的候选基因之一。采用PCR-RFLP技术检测藏猪pGH基因+159~+734 bp片段的DraI、ApaI和MspI酶切多态性。结果显示,藏猪群体中DraI酶切全部切开,无多态;ApaI酶切具有4个等位基因,出现5种基因型,其中CC基因型(299A/432C)频率最高,为0.7857;藏猪群体MspI酶切出现3种基因型,杂合性CT基因型频率最高,为0.5089,等位基因C和T频率分别为0.5580和0.4420。经比较,藏猪pGH基因DraI酶切位点与其他猪种一样无多态性,而ApaI酶切出现了特异的432突变位点,MspI酶切等位基因分布相对均衡,表现出与其他猪种的差异。

关 键 词:藏猪  pGH基因  多态性

Polymorphisms of the Porcine Growth Hormone Gene (pGH) in Tibet Pig
ZHANG Hao,CHAMBA Yangzom,ZHANG Mingming,PENG Junfei,YANG Tao,DAWA Zhuoma,WU Changxin. Polymorphisms of the Porcine Growth Hormone Gene (pGH) in Tibet Pig[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(2)
Authors:ZHANG Hao  CHAMBA Yangzom  ZHANG Mingming  PENG Junfei  YANG Tao  DAWA Zhuoma  WU Changxin
Affiliation:ZHANG Hao1,CHAMBA Yangzom2,ZHANG Mingming3,PENG Junfei1,YANG Tao2,DAWA Zhuoma2 , WU Changxin1(1.College of Animal Science , Technology,China Agricultural University,Beijing 100094,China,2.College of Agriculture , Animal Husb,ry,Tibet University,Linzhi Tibet 860000,3.Institute of Information,Anhui Academy of Agricultrul Sciences,Hefei 230031,China)
Abstract:The porcine growth hormone (pGH) gene is one of important candidate for development and growth trait. In the present study, the polymorphism of fragment +159~+734 of pGH gene was measured with the methods of PCR-RFLP by restriction endonuclease Dra I, Apa I and Msp I. The results showed that the Tibet pig has no diversity for PCR-Dra I-RFLP of pGH gene. There are four alleles and five genotypes for PCR-Apa I-RFLP of pGH gene, and the genotype CC (299A/432C) is predominant (0.7857). The Tibet pig population has three genotypes for PCR-Msp I-RFLP of pGH gene, and the predominant genotype CT is (0.5089). The frequencies of allele C and allele T are 0.5580 and 0.4420 respectively. By comparing with other breeds, the Tibet pig has differential genotypes for Apa I, and the distribution of alleles for Msp I is uniform relatively, which may be the difference of Tibet pig with other pig breeds.
Keywords:PCR-RFLP  Tibet pig  pGH gene  PCR-RFLP  Polymorphism
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